Structure of PDB 6c71 Chain B

Receptor sequence

>6c71B (length=440) Species: 1042876 (Pseudomonas putida S16)

GFDYDVVVVGGGFAGATAARECGLQGYRTLLLEARSRLGGRTFTSRFAGQ
EIEFGGAWVHWLQPHVWAEMQRYGLGVVEDPLTNLDKTLIMYNDGSVESI
SPDEFGKNIRIAFEKLCHDAWEVFPRPHEPMFTERARELDKSSVLDRIKT
LGLSRLQQAQINSYMALYAGETTDKFGLPGVLKLFACGGWNYDAFMDTET
HYRIQGGTIGLINAMLTDSGAEVRMSVPVTAVEQVNGGVKIKTDDDEIIT
AGVVVMTVPLNTYKHIGFTPALSKGKQRFIKEGQLSKGAKLYVHVKQNLG
RVFAFADEQQPLNWVQTHDYSDELGTILSITIARKETIDVNDRDAVTREV
QKMFPGVEVLGTAAYDWTADPFSLGAWAAYGVGQLSRLKDLQAAEGRILF
AGAETSNGWHANIDGAVESGLRAGREVKQLLSLEHHHHHH

3D structure

• PDB: 6c71 Crystallography Coupled with Kinetic Analysis Provides Mechanistic Underpinnings of a Nicotine-Degrading Enzyme.
• Chain: B
• Resolution: 2.649 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H110
• Catalytic site (residue number reindexed from 1): H60
• Enzyme Commision number: 1.4.2.2: nicotine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NCT	B	L217 Y218 E249 W364 T381 W427 A461	L167 Y168 E199 W314 T331 W377 A411
BS02	FAD	B	V59 G60 G61 G62 A64 E83 A84 R85 G90 R91 T92 G105 G106 A107 W108 P278 V279 V308 P309 W417 F422 W427 G452 A453 N462 I463 A466	V9 G10 G11 G12 A14 E33 A34 R35 G40 R41 T42 G55 G56 A57 W58 P228 V229 V258 P259 W367 F372 W377 G402 A403 N412 I413 A416

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:6c71, PDBe:6c71, PDBj:6c71
• PDBsum: 6c71
• PubMed: 29812904
• UniProt: F8G0P2|NICA2_PSEP6 Nicotine dehydrogenase (Gene Name=nicA2)