Structure of PDB 6bsn Chain B

Receptor sequence

>6bsnB (length=973) Species: 224911 (Bradyrhizobium diazoefficiens USDA 110)

IPPPFTAPYAPDDAEIAARLLPASHLSPPQEARIHRTATRLIEAIRKRRL
GGVEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIEDKLGEGDFI
HHETKSTAFLVNASAWALRVIQPGETPDGTIGRLVKRLGAPAVRTATRQA
MRLMGNHFVLGETIEQALERGKPRSGQKTRYSFDMLGEGARTAADARRYF
DAYASAIETIGKAAGNHALPDRPGISVKLSALHPRFEAISRARVMVELVP
QLLDLAQRAKAHDLNFTVDAEEADRLELSLDVIAATLADPSLKGWDGFGL
AIQAYQKRASAVIDYVDALARAHDRKLMVRLVKGAYWDTEIKRAQERGLD
GYPVFTRKAMTDLNYVACASKLLALRPRIFPQFATHNALTVATVLEMAEG
SSGFEFQRLHGMGEALYEQLAKDHADIAYRTYAPVGSHRDLLAYLVRRLL
ENGANSSFVAQAADYRVPVPALLQRPADAIVRPQAAAHPRIPLPCDLFAP
ERRNSRGVEFGARTALDQLLTDVKAEIADATPDQAHAAVAAARAGFAGWS
RTPAGIRAAALEQAAHLLESRSAHFIALLQREGGKTLDDALSELREAADF
CRYYAAQGRKLFGSETAMPGPTGESNALTMRGRGVFVAISPWNFPLAIFL
GQVTAALMAGNSVVAKPAEQTPRIAREAVALLHEAGIPKSALYLVTGDGR
IGAALTAHPDIAGVVFTGSTEVARSINRALAAKDGPIVPLIAETGGINAM
IADATALPEQVADDVVTSAFRSAGQRASALRLLFVQEDVADRMIEMVAGA
ARELKIGDPSDVATHVGPVIDVEAKQRLDAHIARMKTEARLHFAGPAPEG
CFVAPHIFELTEAGQLTEEVFGPILHVVRYRPENLERVLRAIERTGYGLT
LGVHSRIDDSIEAIIDRVQVGNIYVNRNMIGAVVGVQPFGGNGLSGTGPK
AGGPHYLARFATEQTVTINTAAA

3D structure

• PDB: 6bsn Structural Basis for the Substrate Inhibition of Proline Utilization A by Proline.
• Chain: B
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N658 E758 A792 A966
• Catalytic site (residue number reindexed from 1): N643 E743 A777 A951
• Enzyme Commision number: 1.2.1.88: L-glutamate gamma-semialdehyde dehydrogenase.
  1.5.5.2: proline dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FDA	B	D278 A279 Q312 R339 V341 K342 G343 A344 Y345 W346 F364 T365 R366 K367 T370 A393 T394 H395 N396 R417 Y441 S466 F467	D269 A270 Q303 R330 V332 K333 G334 A335 Y336 W337 F355 T356 R357 K358 T361 A384 T385 H386 N387 R408 Y432 S457 F458
BS02	PRO	B	F659 S793 I945 G946 A947 F954	F644 S778 I930 G931 A932 F939
BS03	PRO	B	A973 R974 A976 E978	A958 R959 A961 E963
BS04	PRO	B	G733 F886	G718 F871

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity
• GO:0004657 proline dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
• GO:0042802 identical protein binding

Biological Process

• GO:0006355 regulation of DNA-templated transcription
• GO:0006560 proline metabolic process
• GO:0006561 proline biosynthetic process
• GO:0006562 proline catabolic process
• GO:0010133 proline catabolic process to glutamate

Cellular Component

• GO:0009898 cytoplasmic side of plasma membrane

External links

• PDB: RCSB:6bsn, PDBe:6bsn, PDBj:6bsn
• PDBsum: 6bsn
• PubMed: 29295473
• UniProt: Q89E26