Structure of PDB 6bs1 Chain B

Receptor sequence

>6bs1B (length=430) Species: 9606 (Homo sapiens)

DKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRK
AQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVG
SMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEV
KEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKNSVVFGT
SAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPN
RQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFS
ETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQEL
CSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIA
KELLKTEIDADFPHPLRLRLMGVRISSFPN

3D structure

• PDB: 6bs1 Structural Basis for Human DNA Polymerase Kappa to Bypass Cisplatin Intrastrand Cross-Link (Pt-GG) Lesion as an Efficient and Accurate Extender.
• Chain: B
• Resolution: 3.15 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	I165 I166	I134 I135
BS02	dna	B	R63 D198 E199 K321 S355 G356 I357 G358 V360 T361 R454 T469 R470 A471 T473	R32 D167 E168 K233 S267 G268 I269 G270 V272 T273 R366 T381 R382 A383 T385
BS03	dna	B	F49 M133 S134 M135 A151 P153 F155 I156 S388 S391 R413 K414 S415 S417 V418 E419 T421 R507	F18 M102 S103 M104 A120 P122 F124 I125 S300 S303 R325 K326 S327 S329 V330 E331 T333 R419
BS04	MG	B	R352 I357	R264 I269
BS05	MG	B	D107 M108 D198	D76 M77 D167
BS06	DZ4	B	D107 M108 D109 A110 F111 Y112 S137 T138 Y141 R144 D198	D76 M77 D78 A79 F80 Y81 S106 T107 Y110 R113 D167
BS07	MG	B	D107 D198 E199	D76 D167 E168

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:6bs1, PDBe:6bs1, PDBj:6bs1
• PDBsum: 6bs1
• PubMed: 29715472
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)