Structure of PDB 6brx Chain B

Receptor sequence

>6brxB (length=430) Species: 9606 (Homo sapiens)

DKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRK
AQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVG
SMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEV
KEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKNSVVFGT
SAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPN
RQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFS
ETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQEL
CSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIA
KELLKTEIDADFPHPLRLRLMGVRISSFPN

3D structure

• PDB: 6brx Structural Basis for Human DNA Polymerase Kappa to Bypass Cisplatin Intrastrand Cross-Link (Pt-GG) Lesion as an Efficient and Accurate Extender.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	I165 I166	I134 I135
BS02	dna	B	R63 E199 K321 G356 I357 G358 V360 T361 R454 T469 R470 S472 T473	R32 E168 K233 G268 I269 G270 V272 T273 R366 T381 R382 S384 T385
BS03	dna	B	T44 F49 M133 S134 M135 P153 F155 S388 T390 R413 K414 S415 M416 S417 E419 T421 R507	T13 F18 M102 S103 M104 P122 F124 S300 T302 R325 K326 S327 M328 S329 E331 T333 R419
BS04	MG	B	D107 M108 D198	D76 M77 D167
BS05	0KX	B	D107 M108 A110 F111 Y112 T138 Y141 R144 D198 K328	D76 M77 A79 F80 Y81 T107 Y110 R113 D167 K240
BS06	MG	B	R352 V354 I357	R264 V266 I269

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:6brx, PDBe:6brx, PDBj:6brx
• PDBsum: 6brx
• PubMed: 29715472
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)