Structure of PDB 6awf Chain B

Receptor sequence

>6awfB (length=241) Species: 562 (Escherichia coli)

AEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAPD
LSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIER
DLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCIN
CGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNG
VWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLK

3D structure

• PDB: 6awf New crystal forms of the integral membrane Escherichia coli quinol:fumarate reductase suggest that ligands control domain movement.
• Chain: B
• Resolution: 3.35 Å

Enzymatic activity

• Enzyme Commision number: 1.3.5.1: succinate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FES	B	C57 R58 C62 G63 C65 C77	C57 R58 C62 G63 C65 C77
BS02	F3S	B	C158 F167 P170 C204 T205 F206 C210 A221	C158 F167 P170 C204 T205 F206 C210 A221
BS03	SF4	B	C148 C151 G152 C154 A171 C214 P215	C148 C151 G152 C154 A171 C214 P215

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008177 succinate dehydrogenase (quinone) activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006099 tricarboxylic acid cycle
• GO:0006113 fermentation
• GO:0009061 anaerobic respiration
• GO:0019645 anaerobic electron transport chain
• GO:0044780 bacterial-type flagellum assembly

Cellular Component

• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0045283 fumarate reductase complex

External links

• PDB: RCSB:6awf, PDBe:6awf, PDBj:6awf
• PDBsum: 6awf
• PubMed: 29158068
• UniProt: P0AC47|FRDB_ECOLI Fumarate reductase iron-sulfur subunit (Gene Name=frdB)