Structure of PDB 6at3 Chain B

Receptor sequence
>6at3B (length=534) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
LTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGYERGVLTNLGAVA
VDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETWQHLK
GLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPS
DEELAGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTW
FGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTT
LSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSKEAEPEIYNAIRR
DALLENVTVREDGTIDFDDGSKTENTRVSYPIYHIDNIVKPVSKAGHATK
VIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGITEPTPTFS
ACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTR
AIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQW
QEKAETLAKLFIDNFDKYTDTPAGAALVAAGPKL
3D structure
PDB6at3 Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase.
ChainB
Resolution1.455 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R65 K213 H232 S250 K254 T255 D269 R333
Catalytic site (residue number reindexed from 1) R59 K207 H226 S244 K248 T249 D263 R327
Enzyme Commision number 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 THJ B R74 R79 R68 R73
BS02 THJ B R65 H232 L249 S250 F413 R59 H226 L243 S244 F407
BS03 THJ B S250 G251 G253 K254 T255 T256 S244 G245 G247 K248 T249 T250
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
GO:0005509 calcium ion binding
GO:0005524 ATP binding
GO:0016831 carboxy-lyase activity
GO:0017076 purine nucleotide binding
GO:0046872 metal ion binding
Biological Process
GO:0006094 gluconeogenesis
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:6at3, PDBe:6at3, PDBj:6at3
PDBsum6at3
PubMed30376300
UniProtP22259|PCKA_ECOLI Phosphoenolpyruvate carboxykinase (ATP) (Gene Name=pckA)

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