Structure of PDB 5ztb Chain B

Receptor sequence

>5ztbB (length=311) Species: 262724 (Thermus thermophilus HB27)

VCKVCGQKAQVEMRSRGLALCREHYLDWFVKETERAIRRHRMLLPGERVL
VAVSGGKDSLALWDVLSRLGYQAVGLHIELGIGEYSKRSLEVTQAFARER
GLELLVVDLKEAYGFGVPELARLSGRVACSACGLSKRYIINQVAVEEGFR
VVATGHNLDDEAAVLFGNLLNPTLSRQGPVLPEKPGLAARVKPFYRFSER
EVLSYTLLRGIRYLHEECPNAKGAKSLLYKEALNLVERSMPGAKLRFLDG
FLEKIRPRLDEVALRECERCGYPTTGAVCAFCRMWDAVYRRAKKRKLLPE
EVSFRPRVKPL

3D structure

• PDB: 5ztb The [4Fe-4S] cluster of sulfurtransferase TtuA desulfurizes TtuB during tRNA modification in Thermus thermophilus.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.8.1.15: tRNA-5-methyluridine(54) 2-sulfurtransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	C130 C133 C222 N224	C129 C132 C218 N220
BS02	ZN	B	C3 C6 C22 H25	C2 C5 C21 H24
BS03	ZN	B	C274 C277 C286 C289	C267 C270 C279 C282
BS04	ATP	B	A53 V54 S55 G57 K58 D59 S60 I79 K137 T155 G156 H157	A52 V53 S54 G56 K57 D58 S59 I78 K136 T154 G155 H156

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0005524 ATP binding
• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0002098 tRNA wobble uridine modification
• GO:0002143 tRNA wobble position uridine thiolation
• GO:0008033 tRNA processing
• GO:0034227 tRNA thio-modification

Cellular Component

• GO:0002144 cytosolic tRNA wobble base thiouridylase complex

External links

• PDB: RCSB:5ztb, PDBe:5ztb, PDBj:5ztb
• PDBsum: 5ztb
• PubMed: 32265486
• UniProt: Q72LF3|TTUA_THET2 tRNA-5-methyluridine(54) 2-sulfurtransferase (Gene Name=ttuA)