Structure of PDB 5zbd Chain B

Receptor sequence

>5zbdB (length=411) Species: 243365 (Chromobacterium violaceum ATCC 12472)

HSSDICIVGAGISGLTCASHLLDSPACRGLSLRIFDMQQEAGGRIRSKML
DASIELGAGRYSPQLHPHFQSAMQHYSQKSEVYPFTQLKFKSHVQQKLKR
AMNELSPRLKEHGKESFLQFVSRYQGHDSAVGMIRSMGYDALFLPDISAE
MAYDIVGKHPEIQSVTDNDANQWFAAETGFAGLIQGIKAKVKAAGARFSL
GYRLLSVRTDGDGYLLQLAGDDGWKLEHRTRHLILAIPPSAMAGLNVDFP
EAWSGARYGSLPLFKGFLTYGEPWWLDYKLDDQVLIVDNPLRKIYFKGDK
YLFFYTDSEMANYWRGCVAEGEDGYLEQIRTHLASALGIVRERIPQPLAH
VHKYWAHGVEFCRDIDHPSALSHRDSGIIACSDAYTEHAGWMEGGLLSAR
EASRLLLQRIA

3D structure

• PDB: 5zbd Protein engineering for improving the thermostability of tryptophan oxidase and insights from structural analysis.
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 1.4.3.23: 7-chloro-L-tryptophan oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TRP	B	R71 Y150 H170 Y316 D318 V370 G403 W404	R60 Y139 H159 Y305 D307 V359 G390 W391
BS02	FAD	B	G18 G20 I21 S22 D45 M46 G52 R53 G68 A69 G70 R71 R214 L215 I248 L274 W366 G369 D396 G403 W404 M405	G9 G11 I12 S13 D36 M37 G43 R44 G57 A58 G59 R60 R203 L204 I237 L263 W355 G358 D383 G390 W391 M392

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0017000 antibiotic biosynthetic process

External links

• PDB: RCSB:5zbd, PDBe:5zbd, PDBj:5zbd
• PDBsum: 5zbd
• PubMed: 30053101
• UniProt: Q9S3V1|VIOA_CHRVO Flavin-dependent L-tryptophan oxidase VioA (Gene Name=vioA)