Structure of PDB 5yem Chain B

Receptor sequence

>5yemB (length=678) Species: 85995 (Mycothermus thermophilus)

SPLAAYEVDDSTGYLTSDVGGPIQDQTSLKAGIRGPTLLEDFMFRQKIQH
FDHERVPERAVHARGAGAHGTFTSYADWSNITAASFLNATGKQTPVFVRF
STVAGSRGSADTARDVHGFATRFYTDEGNFDIVGNNIPVFFIQDAIQFPD
LIHSVKPRPDNEIPQAAFAHDSAWDFFSQQPSTMHTLFWAMSGHGIPRSY
RHMDGFGVHTFRFVKDDGSSKLIKWHFKSRQGKASLVWEEAQVLSGKNAD
FHRQDLWDAIESGNGPEWDVCVQIVDESQAQAFGFDLLDPTKIIPEEYAP
LTKLGLLKLDRNPTNYFAETEQVMFQPGHIVRGIDFTEDPLLQGRLFSYL
DTQLNRNGGPNFEQLPINMPRVPIHNNNRDGAGQMFIHRNKYPYTPNTLN
SGYPRQANQNAGRGFFTAPGRTASGALVREVSPTFNDHWSQPRLFFNSLT
PVEQQFLVNAMRFEISLVKSEEVKKNVLTQLNRVSHDVAVRVAAAIGLGA
PDADDTYYHNNKTAGVSIVGSGPLPTIKTLRVGILATTSESSALDQAAQL
RTRLEKDGLVVTVVAETLREGVDQTYSTADATGFDGVVVVDGAAALFAST
ASSPLFPTGRPLQIFVDAYRWGKPVGVCGGKSSEVLDAADVPEDGDGVYS
EESVDMFVEEFEKGLATFRFTDRFALDS

3D structure

• PDB: 5yem CATPO mutant - T188F
• Chain: B
• Resolution: 1.49 Å

Enzymatic activity

• Enzyme Commision number: 1.11.1.6: catalase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	F436 T437	F416 T417
BS02	CA	B	R178 H190 D191 S192	R158 H170 D171 S172
BS03	CA	B	S255 F476	S235 F456
BS04	HEM	B	R79 V81 H82 R119 V153 G154 N155 F160 A165 F168 V228 H229 F345 L361 R365 S368 Y369 T372 Q373 R376	R59 V61 H62 R99 V133 G134 N135 F140 A145 F148 V208 H209 F325 L341 R345 S348 Y349 T352 Q353 R356

Gene Ontology

Molecular Function

• GO:0004096 catalase activity
• GO:0004601 peroxidase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006979 response to oxidative stress
• GO:0042744 hydrogen peroxide catabolic process
• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5yem, PDBe:5yem, PDBj:5yem
• PDBsum: 5yem
• PubMed: 38265876
• UniProt: M4GGR5