Structure of PDB 5xbz Chain B

Receptor sequence

>5xbzB (length=705) Species: 4839 (Rhizomucor miehei)

GDDLFVPVSNFDPKSIFPEIKHPFEPMYANTENGKIVPTNSWISNLFYPS
ADNLAPTTPDPYTLRLLDGYGGNPGLTIRQPSAKVLGSYPPTDAGYMINS
VVVDLRLTSSEWSDVVPDRQVTDWDHLSANLRLSTPQDSNSYIDFPIVRG
MAYITANYNNLTPQFLSQHAIISVEADEFSGRKFKITMNDDPTSTFIIYS
LGDKPLELRKQDNSNLVASKPYTGVIRVAKLPAPEFETLLDASRAVWPTG
GDISARSDDNNGASYTIKWKTNSNEAPLLTYAYAHHLTSIDDSNVKRTDM
TLQSATKGPMTALVGNEWTLRETELSPVEWLPLQAAPNPTTINEIMTEIN
KDIASNYTQETAKEDNYFSGKGLQKFAMLALILNKSDQTQLRNPELAQIA
LDKLKAAFLPYLQNEQADPFRYDTLYKGIVAKAGLPTSMGGTDDLSAEFG
HSYYSDHHYHQGYFVVTAAIIHHLDPTWNADRLKAWTEALIRDVNNANDG
DEYFAAFRNWDWFAGHSWAGGIKPDGALDGRDQESVPESVNFYWGAKLWG
LATGNTPLTKLASLQLAVTKRTTYEYFWMLDGNKNRPENIVRNKVIGIYF
EQKTDYTTYFGRFLEYIHGIQQLPMTPELMEYIRTPEFVSQEWDEKLGAI
APTVQSPWAGVLYLNYAIINPAEAYPALRKVQMDDGQTRSYSLYLTATRP
HFFRR

3D structure

• PDB: 5xbz Catalytic mechanism of glycoside hydrolase family 81 beta-1,3-glucanase
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.39: glucan endo-1,3-beta-D-glucosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	K542 D551 Y628	K523 D532 Y609
BS02	BGC	B	Y386 F468 Y478 E553 Y628	Y367 F449 Y459 E534 Y609
BS03	BGC	B	Y386 F387 K390 F629	Y367 F368 K371 F610
BS04	BGC	B	D548 K622	D529 K603
BS05	BGC	B	D624 T626 T627	D605 T607 T608
BS06	BGC	B	T627 Y628 G630	T608 Y609 G611
BS07	BGC	B	D548 K622	D529 K603
BS08	BGC	B	K622 D624	K603 D605
BS09	BGC	B	Y93 D624	Y89 D605

Gene Ontology

Molecular Function

• GO:0052861 endo-1,3(4)-beta-glucanase activity

External links

• PDB: RCSB:5xbz, PDBe:5xbz, PDBj:5xbz
• PDBsum: 5xbz
• UniProt: A0A023I7E1|ENG1_RHIMI Glucan endo-1,3-beta-D-glucosidase 1 (Gene Name=ENG1)