Structure of PDB 5wp5 Chain B

Receptor sequence

>5wp5B (length=462) Species: 3702 (Arabidopsis thaliana)

EERDIQKSYWMEHSEVLSLIPPYEGKSVLELGAGIGRFTGELAQKAGEVI
ALDFIESAIQKNESVNGHYKNIKFMCADVTSPDLKIKDGSIDLIFSNWLL
MYLSDKEVELMAERMIGWVKPGGYIFFRESCFHQSGDSKRKSNPTHYREP
RFYTKVFQECQTRDASGNSFELSMVGCKCIGAYVKNKKNQNQICWIWQKV
SVENDKDFQRFLDNVQYKSSGILRYERVFGEGYVSTGGFETTKEFVAKMD
LKPGQKVLDVGCGIGGGDFYMAENFDVHVVGIDLSVNMISFALERAIGLK
CSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGG
KVLITDYCRSAETPSPEFAEYIKQRGYDLHDVQAYGQMLKDAGFDDVIAE
DRTDQFVQVLRRELEKVEKEKEEFISDFSEEDYNDIVGGWSAKLERTASG
EQKWGLFIADKK

3D structure

• PDB: 5wp5 Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation.
• Chain: B
• Resolution: 1.5 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.103: phosphoethanolamine N-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAH	B	W16 G61 R66 F67 D82 F83 D107 V108 N126 W127	W10 G32 R37 F38 D53 F54 D78 V79 N97 W98	MOAD: Kd=4.6uM
BS02	SAH	B	V263 S264 G290 G292 D312 L313 M317 D338 C339 R355 T357 H360	V234 S235 G261 G263 D283 L284 M288 D309 C310 R326 T328 H331	MOAD: Kd=4.6uM

Gene Ontology

Molecular Function

• GO:0000234 phosphoethanolamine N-methyltransferase activity
• GO:0003729 mRNA binding
• GO:0008168 methyltransferase activity

Biological Process

• GO:0006656 phosphatidylcholine biosynthetic process
• GO:0008654 phospholipid biosynthetic process
• GO:0009058 biosynthetic process
• GO:0032259 methylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5wp5, PDBe:5wp5, PDBj:5wp5
• PDBsum: 5wp5
• PubMed: 29084845
• UniProt: Q944H0|PEAM2_ARATH Phosphoethanolamine N-methyltransferase 2 (Gene Name=NMT2)