Structure of PDB 5wdg Chain B

Receptor sequence

>5wdgB (length=554) Species: 573 (Klebsiella pneumoniae) [Search protein sequence]

PRGSHMDKQYPVRQWAHGADLVVSQLEAQGVRQVFGIPGAKIDKVFDSLL
DSSIRIIPVRHEANAAFMAAAVGRITGKAGVALVTSGPGCSNLITGMATA
NSEGDPVVALGGAVKRADKAKQVHQSMDTVAMFSPVTKYAIEVTAPDALA
EVVSNAFRAAEQGRPGSAFVSLPQDVVDGPVSGKVLPASGAPQMGAAPDD
AIDQVAKLIAQAKNPIFLLGLMASQPENSKALRRLLETSHIPVTSTYQAA
GAVNQDNFSRFAGRVGLFNNQAGDRLLQLADLVICIGYSPVEYEPAMWNS
GNATLVHIDVLPAYEERNYTPDVELVGDIAGTLNKLAQNIDHRLVLSPQA
AEILRDRQHQRRGAQLNQFALHPLRIVRAMQDIVNSDVTLTVDMGSFHIW
IARYLYSFRARQVMISNGQQTMGVALPWAIGAWLVNPERKVVSVSGDGGF
LQSSMELETAVRLKANVLHLIWVDNGYNMVAIQEEKKYQRLSGVEFGPMD
FKAYAESFGAKGFAVESAEALEPTLRAAMDVDGPAVVAIPVDYRDNPLLM
GQLH

3D structure

PDB

5wdg Acetolactate Synthase from Klebsiella pneumoniae in Complex with Mechanism-Based Inhibitor

Chain

Resolution

2.12 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	I32 G34 A35 K36 I37 E57 T80 H119 Q120 S121 Q169 L262 E289 M394 Q420 M422 D447 D474 G476 Y477 M479 V480 Q483 Y543
Catalytic site (residue number reindexed from 1)	I37 G39 A40 K41 I42 E62 T85 H124 Q125 S126 Q174 L267 E294 M394 Q420 M422 D447 D474 G476 Y477 M479 V480 Q483 Y543
Enzyme Commision number	2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	MG	B	D447 D474 G476	D447 D474 G476
BS02	MG	B	S129 T132	S134 T137
BS03	MG	B	L44 S47 I49	L49 S52 I54
BS04	A4Y	B	M394 S396 F397 Q420 M422 G448 G449 D474 G476 Y477 N478 M479 V480 Y543	M394 S396 F397 Q420 M422 G448 G449 D474 G476 Y477 N478 M479 V480 Y543
BS05	PYR	B	Y488 R490	Y488 R490

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0003984	acetolactate synthase activity
GO:0016740	transferase activity
GO:0030976	thiamine pyrophosphate binding
GO:0046872	metal ion binding
GO:0050660	flavin adenine dinucleotide binding

	Biological Process
GO:0009097	isoleucine biosynthetic process
GO:0009099	L-valine biosynthetic process
GO:0019752	carboxylic acid metabolic process
GO:0034077	butanediol metabolic process

	Cellular Component
GO:0005948	acetolactate synthase complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:5wdg, PDBe:5wdg, PDBj:5wdg
PDBsum	5wdg
PubMed
UniProt	P27696\|ILVB_KLEPN Acetolactate synthase, catabolic (Gene Name=budB)

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