Structure of PDB 5v9j Chain B

Receptor sequence

>5v9jB (length=259) Species: 9606 (Homo sapiens)

ERIVSRDIARGYERIPIPCVNAVDSEPCPSNYKYVSQNCVTSPMNIDRNI
THLQYCVCIDDCSSSNCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECN
HACSCWRNCRNRVVQNGLRARLQLYRTRDMGWGVRSLQDIPPGTFVCEYV
GELISDSEADVREEDSYLFDLDNKGEVYCIDARFYGNVSRFINHHCEPNL
VPVRVFMAHQDLRFPRIAFFSTRLIEAGEQLGFDYGERFWDIKGKLFSCR
CGSPKCRHS

3D structure

• PDB: 5v9j Crystal structure of catalytic domain of GLP with MS0105
• Chain: B
• Resolution: 1.74 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y1155 Y1242
• Catalytic site (residue number reindexed from 1): Y149 Y235
• Enzyme Commision number: 2.1.1.-
  2.1.1.367: [histone H3]-lysine(9) N-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAM	B	M1136 W1138 S1172 Y1173 R1197 F1198 N1200 H1201 Y1242 F1246 F1254 C1256 R1257	M130 W132 S166 Y167 R190 F191 N193 H194 Y235 F239 F247 C249 R250
BS02	90P	B	D1162 A1165 D1166 R1168 D1171 L1174 D1176 V1184 C1186 R1245 F1246 I1249 K1250	D156 A159 D160 R162 D165 L168 D170 V177 C179 R238 F239 I242 K243
BS03	ZN	B	C1062 C1075 C1105 C1109	C56 C69 C99 C103
BS04	ZN	B	C1068 C1105 C1111 C1115	C62 C99 C105 C109
BS05	ZN	B	C1062 C1064 C1068 C1073	C56 C58 C62 C67
BS06	ZN	B	C1203 C1256 C1258 C1263	C196 C249 C251 C256

Gene Ontology

Molecular Function

• GO:0002039 p53 binding
• GO:0008270 zinc ion binding
• GO:0016279 protein-lysine N-methyltransferase activity
• GO:0042054 histone methyltransferase activity
• GO:0046974 histone H3K9 methyltransferase activity

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:5v9j, PDBe:5v9j, PDBj:5v9j
• PDBsum: 5v9j
• UniProt: Q9H9B1|EHMT1_HUMAN Histone-lysine N-methyltransferase EHMT1 (Gene Name=EHMT1)