Structure of PDB 5v56 Chain B

Receptor sequence

>5v56B (length=634) Species: 882,9606

PPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVL
WSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVE
RERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCMVPLVRTDNPKSWYE
DVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRN
SNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTS
NETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTS
YFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPI
GLVLIVGGYFLIRGVMTLFSIKSNHAKALIVYGSTTGNTEYTAETIAREL
ADAGYEVDSRDAASVEAGGLFEGFDLVLLGCSTWGDDSIELQDDFIPLFD
SLEETGAQGRKVACFGCGDSSWEYFCGAVDAIEEKLKNLGAEIVQDGLRI
DGDPRAARDDIVGWAHDVRGAIKINETMLRLGIFGFLAFGFVLITFSCHF
YDFFNQAEWERSFRDYVLCQANVTIGLPDCEIKNRPSLLVEKINLFAMFG
TGIAMSTWVWTKATLLIWRRTWCRLTGQSDDHHH

3D structure

• PDB: 5v56 Crystal structure of a multi-domain human smoothened receptor in complex with a super stabilizing ligand.
• Chain: B
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	836	B	N219 M230 W281 M301 D384 S387 F391 Y394 K395 R400 Q477 W480 E481 F484 P513 E518 N521	N161 M172 W223 M243 D326 S329 F333 Y336 K337 R342 Q556 W559 E560 F563 P586 E591 N594
BS02	FMN	B	S1010 T1011 T1012 G1013 N1014 T1015 S1058 T1059 W1060 G1061 C1093 G1094 D1095 W1098 Y1100 F1101 C1102	S384 T385 T386 G387 N388 T389 S432 T433 W434 G435 C467 G468 D469 W472 Y474 F475 C476

Gene Ontology

Molecular Function

• GO:0004888 transmembrane signaling receptor activity
• GO:0009055 electron transfer activity
• GO:0010181 FMN binding

Biological Process

• GO:0007166 cell surface receptor signaling pathway

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:5v56, PDBe:5v56, PDBj:5v56
• PDBsum: 5v56
• PubMed: 28513578
• UniProt: P00323|FLAV_NITV2 Flavodoxin (Gene Name=DVU_2680);
  Q99835|SMO_HUMAN Protein smoothened (Gene Name=SMO)