Structure of PDB 5ugh Chain B

Receptor sequence
>5ughB (length=365) Species: 9606 (Homo sapiens) [Search protein sequence]
AFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVA
KTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVAL
EQQSPDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRP
DSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIK
AVVPAKYLDEDTIYHLQPSGRFVIGGPDAGLTGRKIIVDTYGGWGAHGGG
AFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSI
SIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHF
GRDSFPWEVPKKLKY
3D structure
PDB5ugh Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A.
ChainB
Resolution2.062 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H29 D31 K32 E57 E70 K181 F250 D258 A259 R264 K265 K285 K289 D291
Catalytic site (residue number reindexed from 1) H19 D21 K22 E47 E60 K153 F222 D228 A229 R234 K235 K255 K259 D261
Enzyme Commision number 2.5.1.6: methionine adenosyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 8AJ B L315 S331 I332 F333 E342 L285 S301 I302 F303 E312 MOAD: Kd=170nM
BS02 8AJ B F18 F20 Q190 W274 F8 F10 Q162 W244 MOAD: Kd=170nM
Gene Ontology
Molecular Function
GO:0004478 methionine adenosyltransferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0036094 small molecule binding
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0006556 S-adenosylmethionine biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0034214 protein hexamerization
GO:0051291 protein heterooligomerization
GO:0061431 cellular response to methionine
GO:1904263 positive regulation of TORC1 signaling
GO:1990830 cellular response to leukemia inhibitory factor
Cellular Component
GO:0005829 cytosol
GO:0048269 methionine adenosyltransferase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5ugh, PDBe:5ugh, PDBj:5ugh
PDBsum5ugh
PubMed28553945
UniProtP31153|METK2_HUMAN S-adenosylmethionine synthase isoform type-2 (Gene Name=MAT2A)

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