Structure of PDB 5u63 Chain B

Receptor sequence

>5u63B (length=319) Species: 71421 (Haemophilus influenzae Rd KW20)

SNAMSDIKHAKLLILGSGPAGYTAAIYAARANLKPVLVTGLQQGGQLTTT
DEIENWPGDFEMTTGSGLMQRMLQHAEKFETEIVFDHINRVDLSSRPFKL
FGDVQNFTCDALIIATGASARYIGLPSEENYKGRGVSACATCDGFFYRNK
PVGVIGGGNTAVEEALYLANIASTVHLIHRRDSFRAEKILIDRLYKKVEE
GKIVLHTDRTLDEVLGDNMGVTGLRLANTKTGEKEELKLDGLFVAIGHSP
NTEIFQGQLELNNGYIVVKSGLDGNATATSVEGVFAAGDVMDHNYRQAIT
SAGTGCMAALDAERYLDAQ

3D structure

• PDB: 5u63 Crystal structure of putative thioredoxin reductase from Haemophilus influenzae
• Chain: B
• Resolution: 1.99 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C136 C139 D140
• Catalytic site (residue number reindexed from 1): C139 C142 D143
• Enzyme Commision number: 1.8.1.9: thioredoxin-disulfide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	G13 S14 G15 P16 A17 T36 G37 Q39 G42 Q43 L44 T47 N52 H84 I85 A112 T113 G114 H245 G285 D286 R293 Q294 A295 S298	G16 S17 G18 P19 A20 T39 G40 Q42 G45 Q46 L47 T50 N55 H87 I88 A115 T116 G117 H248 G288 D289 R296 Q297 A298 S301
BS02	NAP	B	R118 G154 H176 R177 R178 I243 G244 H245 R293	R121 G157 H179 R180 R181 I246 G247 H248 R296

Gene Ontology

Molecular Function

• GO:0004791 thioredoxin-disulfide reductase (NADPH) activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0019430 removal of superoxide radicals
• GO:0045454 cell redox homeostasis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5u63, PDBe:5u63, PDBj:5u63
• PDBsum: 5u63
• UniProt: P43788|TRXB_HAEIN Thioredoxin reductase (Gene Name=trxB)