Structure of PDB 5tj9 Chain B

Receptor sequence

>5tj9B (length=494) Species: 353152 (Cryptosporidium parvum Iowa II)

KMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGS
LHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAW
KNETIEDYWVCLNDAMTWRNPNDKDKICGPNLIVDDGGDATLILHEGVKA
EIEYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGM
LKDLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCR
QSLLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDP
ICALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYI
ANIGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRL
VNLGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSK
ELDEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5tj9 2.6 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Aristeromycin and NAD
• Chain: B
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H52 S75 S80 D136 E210 N235 K240 D244 N245 C249 H355 H409 S417 Q421
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	K489 Y493	K488 Y492
BS02	7CY	B	H53 T55 E57 D137 E211 T212 K241 D245 H356 H410 M415	H52 T54 E56 D136 E210 T211 K240 D244 H355 H409 M414
BS03	NAD	B	T212 T213 T214 N246 G277 E278 V279 T297 E298 I299 T331 N333 I354 H356 N403 H410	T211 T212 T213 N245 G276 E277 V278 T296 E297 I298 T330 N332 I353 H355 N402 H409

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5tj9, PDBe:5tj9, PDBj:5tj9
• PDBsum: 5tj9
• UniProt: Q5CPH1