Structure of PDB 5tf9 Chain B

Receptor sequence
>5tf9B (length=266) Species: 9606 (Homo sapiens) [Search protein sequence]
ELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDR
KLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMT
SGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNI
FITGPTGSVKIGDLGLATLKGTPEFMAPEMYAAAYDESVDVYAFGMCMLE
MATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNK
DERYSIKDLLNHAFFQ
3D structure
PDB5tf9 Discovery and Characterization of Allosteric WNK Kinase Inhibitors.
ChainB
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D349 K351 D353 N354 D368 K375 T386
Catalytic site (residue number reindexed from 1) D144 K146 D148 N149 D163 K170 T172
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 7AV B F265 E268 V281 F283 L299 D368 L369 G370 A372 F60 E63 V76 F78 L94 D163 L164 G165 A167 MOAD: Kd=4.4uM
BindingDB: IC50=420nM
BS02 ANP B G228 R229 G230 K233 L303 M304 K351 G23 R24 G25 K28 L98 M99 K146
BS03 MN B N354 D368 N149 D163
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
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Biological Process
External links
PDB RCSB:5tf9, PDBe:5tf9, PDBj:5tf9
PDBsum5tf9
PubMed27712055
UniProtQ9H4A3|WNK1_HUMAN Serine/threonine-protein kinase WNK1 (Gene Name=WNK1)

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