Structure of PDB 5tf0 Chain B

Receptor sequence

>5tf0B (length=733) Species: 471870 (Bacteroides intestinalis DSM 17393)

PQDMDRFIDALMKKMTVEEKIGQLNLPVSGSDVAKKIEQGLVGGLLNLKG
VEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAI
RKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIA
KAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNR
MFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFD
GFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSI
DEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRD
AARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGT
WSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGR
SLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPD
VQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAY
AIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFR
SNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGSVTAMVTVTNTGKY
DGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLR
FYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL

3D structure

• PDB: 5tf0 Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis
• Chain: B
• Resolution: 2.2021 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D292 S523
• Catalytic site (residue number reindexed from 1): D256 S487
• Enzyme Commision number: 3.2.1.21: beta-glucosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	D701 V703	D662 V664

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0008422 beta-glucosidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0009251 glucan catabolic process

External links

• PDB: RCSB:5tf0, PDBe:5tf0, PDBj:5tf0
• PDBsum: 5tf0
• UniProt: B3C777