Structure of PDB 5t03 Chain B

Receptor sequence

>5t03B (length=675) Species: 83334 (Escherichia coli O157:H7)

IEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVA
ATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNG
KLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNL
QEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLIKN
KHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTFK
GQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGA
VALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAA
SGRQTVDAALAAAQTNAAAKFNFTERDLTRDVDFNIKGDDVIVFLHIQKT
GGTTFGRHLVRNIRLEQPCDCKAKKCTCHRPGKQESWLFSRFSTGWSCGL
HADWTELTNCVPVIMDRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLH
MCDGRSPTQDELPTCYNGDDWSGVTLHDFMDCPSNLANNRQVRMLADLSL
VGCYNLSTMNESERNPILLASAKSNLKNMAFYGLTEFQRKTQYLFERTFH
LRFISAFTQINSTRAANVELRDDMRSRIEQLNMLDMQLYEFAKDLFLQRY
QFVRQRERQEERLKRREERRWIRER

3D structure

• PDB: 5t03 Structure Based Substrate Specificity Analysis of Heparan Sulfate 6-O-Sulfotransferases.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 2.8.2.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BDP	B	R1148 W1153 L1157	R441 W446 L450
BS02	GNS	B	R1148 H1158 H1203 A1208 W1210	R441 H451 H488 A493 W495
BS03	GLC	B	D14 K15 E111 Y155 W230	D13 K14 E110 Y154 W229
BS04	GLC	B	W62 D65 E153 P154 Y155 W340	W61 D64 E152 P153 Y154 W339
BS05	GLC	B	E44 R66 E153 P154 W340 R344	E43 R65 E152 P153 W339 R343
BS06	GLC	B	K42 E44 E45 Y341 R344	K41 E43 E44 Y340 R343
BS07	A3P	B	H1101 K1104 G1106 G1107 T1108 T1109 R1191 S1199 T1300 Q1303 I1325 T1328 R1329 A1330	H396 K399 G401 G402 T403 T404 R476 S484 T585 Q588 I610 T613 R614 A615

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008146 sulfotransferase activity
• GO:0015144 carbohydrate transmembrane transporter activity
• GO:1901982 maltose binding

Biological Process

• GO:0006974 DNA damage response
• GO:0008643 carbohydrate transport
• GO:0015768 maltose transport
• GO:0034219 carbohydrate transmembrane transport
• GO:0034289 detection of maltose stimulus
• GO:0042956 maltodextrin transmembrane transport
• GO:0055085 transmembrane transport
• GO:0060326 cell chemotaxis

Cellular Component

• GO:0016020 membrane
• GO:0030288 outer membrane-bounded periplasmic space
• GO:0042597 periplasmic space
• GO:0043190 ATP-binding cassette (ABC) transporter complex
• GO:0055052 ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
• GO:1990060 maltose transport complex

External links

• PDB: RCSB:5t03, PDBe:5t03, PDBj:5t03
• PDBsum: 5t03
• PubMed: 28103688
• UniProt: P0AEX9|MALE_ECOLI Maltose/maltodextrin-binding periplasmic protein (Gene Name=malE)