Structure of PDB 5sxs Chain B

Receptor sequence

>5sxsB (length=713) Species: 320372 (Burkholderia pseudomallei 1710b)

GTSNRDWWPNQLDLSILHRHSSLSDPMGKDFNYAQAFEKLDLAAVKRDLH
ALMTTSQDWWPADFGHYGGLFIRMAWHSAGTYRTADGRGGAGEGQQRFAP
LNSWPDNANLDKARRLLWPIKQKYGRAISWADLLILTGNVALESMGFKTF
GFAGGRADTWEPEDVYWGSEKIWLELSGGPNSRYSGDRQLENPLAAVQMG
LIYVNPEGPDGNPDPVAAARDIRDTFARMAMNDEETVALIAGGHTFGKTH
GAGPASNVGAEPEAAGIEAQGLGWKSAYRTGKGADAITSGLEVTWTTTPT
QWSHNFFENLFGYEWELTKSPAGAHQWVAKGADAVIPDAFDPSKKHRPTM
LTTDLSLRFDPAYEKISRRFHENPEQFADAFARAWFKLTHRDMGPRARYL
GPEVPAEVLLWQDPIPAVDHPLIDAADAAELKAKVLASGLTVSQLVSTAW
AAASTFRGSDKRGGANGARIRLAPQKDWEANQPEQLAAVLETLEAIRTAF
NGAQRGGKQVSLADLIVLAGCAGVEQAAKNAGHAVTVPFAPGRADASQEQ
TDVESMAVLEPVADGFRNYLKGKYRVPAEVLLVDKAQLLTLSAPEMTVLL
GGLRVLGANVGQSRHGVFTAREQALTNDFFVNLLDMGTEWKPTAADADVF
EGRDRATGELKWTGTRVDLVFGSHSQLRALAEVYGSADAQEKFVRDFVAV
WNKVMNLDRFDLA

3D structure

• PDB: 5sxs Isonicotinic acid hydrazide conversion to Isonicotinyl-NAD by catalase-peroxidases.
• Chain: B
• Resolution: 1.887 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R108 H112 H279 W330 D389
• Catalytic site (residue number reindexed from 1): R73 H77 H244 W295 D354
• Enzyme Commision number: 1.11.1.21: catalase peroxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	G104 L105 I107 W111 V239 P241 L274 I275 G278 H279 K283 T284 H285 T323 S324 W330 W420	G69 L70 I72 W76 V204 P206 L239 I240 G243 H244 K248 T249 H250 T288 S289 W295 W385
BS02	OXY	B	R108 H112	R73 H77
BS03	OXY	B	W111 H112	W76 H77
BS04	NIZ	B	R123 E128 E198 G493 S494 Q622	R88 E93 E163 G458 S459 Q587
BS05	AMP	B	D222 A252 R255	D187 A217 R220

Gene Ontology

Molecular Function

• GO:0004096 catalase activity
• GO:0004601 peroxidase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006979 response to oxidative stress
• GO:0042744 hydrogen peroxide catabolic process
• GO:0070301 cellular response to hydrogen peroxide
• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5sxs, PDBe:5sxs, PDBj:5sxs
• PDBsum: 5sxs
• PubMed: 20554537
• UniProt: Q3JNW6|KATG_BURP1 Catalase-peroxidase (Gene Name=katG)