Structure of PDB 5pzn Chain B

Receptor sequence

>5pznB (length=540) Species: 333284 (Hepatitis C virus (isolate Con1))

SMSYTWTGALITPCYATTSRSASLRQKKVTFDRLQVLDDHYRDVLKEMKA
KASTVKAKLLSVEEACKLTPPHSARSKFGYGAKDVRNLSSKAVNHIRSVW
KDLLEDTETPIDTTIMAKNEVFCVQPGGRKPARLIVFPDLGVRVCEKMAL
YDVVSTLPQAVMGSSYGFQYSPGQRVEFLVNAWKAKKCPMGFAYDTRCFD
STVTENDIRVEESIYQCCDLAPEARQAIRSLTERLYIGGPLTNSKGQNCG
YRRCRASGVLTTSCGNTLTCYLKAAAACRAAKLQDCTMLVCGDDLVVICE
SAGTQEDEASLRAFTEAMTRYSAPPGDPPKPEYDLELITSCSSNVSVAHD
ASGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMYAPTLWARMIL
MTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIQRLHGLSAFSLH
SYSPGEINRVASCLRKLGVPPLRVWRHRARSVRARLLSQGGRAATCGKYL
FNWAVRTKLKLTPIPAASQLDLSSWFVAGYSGGDIYHSLS

3D structure

• PDB: 5pzn Discovery of a Hepatitis C Virus NS5B Replicase Palm Site Allosteric Inhibitor (BMS-929075) Advanced to Phase 1 Clinical Studies.
• Chain: B
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	23E	B	L392 A395 A396 H428 L492 G493 V494 P495 R498 V499 W500 R503	L367 A370 A371 H403 L467 G468 V469 P470 R473 V474 W475 R478
BS02	8XP	B	P197 R200 L204 L314 V321 I363 S365 C366 S368 M414 Y448 Y452	P172 R175 L179 L289 V296 I338 S340 C341 S343 M389 Y423 Y427

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:5pzn, PDBe:5pzn, PDBj:5pzn
• PDBsum: 5pzn
• PubMed: 28430437
• UniProt: Q9WMX2|POLG_HCVCO Genome polyprotein