Structure of PDB 5obp Chain B

Receptor sequence

>5obpB (length=418) Species: 66821 (Sulfurospirillum multivorans)

AEIRQQFAMTAGSPIIVNDKLERYAEVRTAFTHPTSFFKPNYKGEVKPWF
LSAYDEKVRQIENGENGPKMKAKNVGEARAGRALEAAGWTLDINYGNIYP
NRFFMLWSGETMTNTQLWAPVGLDRRPPDTTDPVELTNYVKFAARMAGAD
LVGVARLNRNWVYSEAVTIPADVPYEQSLHKEIEKPIVFKDVPLPIETDD
ELIIPNTCENVIVAGIAMNREMMQTAPNSMACATTAFCYSRMCMFDMWLC
QFIRYMGYYAIPSCNGVGQSVAFAVEAGLGQASRMGACITPEFGPNVRLT
KVFTNMPLVPDKPIDFGVTEFCETCKKCARECPSKAITEGPRTFEGRSIH
NQSGKLQWQNDYNKCLGYWPESGGYCGVCVAVCPFTKNITEVWDGKINTY
GLDADHFRDTVSFRKDRV

3D structure

• PDB: 5obp Selective Utilization of Benzimidazolyl-Norcobamides as Cofactors by the Tetrachloroethene Reductive Dehalogenase of Sulfurospirillum multivorans.
• Chain: B
• Resolution: 1.593 Å

Enzymatic activity

• Enzyme Commision number: 1.21.99.5: tetrachloroethene reductive dehalogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	R291 M292 I296 F328 C329 C332 C335 C390 P391 F392	R284 M285 I289 F321 C322 C325 C328 C383 P384 F385
BS02	SF4	B	C339 P340 S341 I344 C372 C383 C386	C332 P333 S334 I337 C365 C376 C379
BS03	9QQ	B	Y31 T36 A37 F38 Y170 Y246 M249 N272 Q276 S277 V278 A289 M292 G293 A294 C295 V304 R305 L306 H357 N358 Q359 K362 Q364 Y369 C372 W376 Y382 C386	Y24 T29 A30 F31 Y163 Y239 M242 N265 Q269 S270 V271 A282 M285 G286 A287 C288 V297 R298 L299 H350 N351 Q352 K355 Q357 Y362 C365 W369 Y375 C379

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:5obp, PDBe:5obp, PDBj:5obp
• PDBsum: 5obp
• PubMed: 29378885
• UniProt: O68252|PCEA_SULMU Tetrachloroethene reductive dehalogenase (Gene Name=pceA)