Structure of PDB 5n9x Chain B

Receptor sequence

>5n9xB (length=486) Species: 1931 (Streptomyces sp.)

FGATLVDLFSRAAMEMPDRTALHIDDEKISYGLLHSWAEGLADLLHDAGV
RKGDRVALRMPPGANAIAAMLGILRAGAAYVPLDIRNPPARNAFIVTDSQ
VVALVGDPTGPLVTEENVAALRDREGPERPGPQDVAYIIYTSGTTGRPKG
VPVRHGNVTALFEACSRLFSFSADDRWLLFHSMAFDFSVWEIWGALSTGA
ELVVLPTARTPVETARVVRDRGITVLNQTPTAFGALTTAVLGEGIDLPEL
RYVVFGGEKLTPAVVRPWAKRFGLDRPHLINMYGITETTVHATFHRLTED
DLAAEDSVIGRPLPGFTHRIVTEDGRDAATGEPGELWLAGPQVSEGYLNR
PELTAERFTTGPPRYYHSGDLVSRRAGGDLVYQGRADLQVKLRGHRIELS
DVEAAVRTHPAVVDAVVWVHEFAPGDSRLVCAYTAQADARALRAHVKTVL
PSYMQPSQYLALPELPRTINGKADRASVARAFDERR

3D structure

• PDB: 5n9x Structure of the adenylation domain Thr1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces sp. OH-5093-protein flexibility and molecular bases of substrate specificity.
• Chain: B
• Resolution: 2.396 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T167 L187 T314 E315 K427 R432 K515
• Catalytic site (residue number reindexed from 1): T141 L161 T286 E287 K391 R396 K472

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	8QN	B	T167 D212 F213 G285 E286 K287 N309 M310 Y311 G312 I313 T314 V318 D406 Y418 R421 N513 K515	T141 D186 F187 G257 E258 K259 N281 M282 Y283 G284 I285 T286 V290 D370 Y382 R385 N470 K472

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0005524 ATP binding
• GO:0031177 phosphopantetheine binding

Biological Process

• GO:0043041 amino acid activation for nonribosomal peptide biosynthetic process
• GO:0044550 secondary metabolite biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5n9x, PDBe:5n9x, PDBj:5n9x
• PDBsum: 5n9x
• PubMed: 28704585
• UniProt: H6SG27