Structure of PDB 5n61 Chain B

Receptor sequence
>5n61B (length=1177) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
TADFRTLERESRFINPPKDKSAFPLLQEAVQPHIGSFNALTEGPDGGLLN
LGVKDIGEKVIFDGKPLNSNSGYLGNKLSVSVEQVSIAKPMSNDGVSSAV
ERKVYPSESRQRLTSYRGKLLLKLKWSVNNGEENLFEVRDCGGLPVMLQS
NRCHLNKMSPYELVQHKEESDEIGGYFIVNGIEKLIRMLIVQRRNHPMAI
IRPSFANRGASYSHYGIQIRSVRPDQTSQTNVLHYLNDGQVTFRFSWRKN
EYLVPVVMILKALCHTSDREIFDGIIGNDVKDSFLTDRLELLLRGFKKRY
PHLQNRTQVLQYLGDKFRVVFQASPDQSDLEVGQEVLDRIVLVHLGKDGS
QDKFRMLLFMIRKLYSLVAGECSPDNPDATQHQEVLLGGFLYGMILKEKI
DEYLQNIIAQVRMDINRGMAINFKDKRYMSRVLMRVNENIGSKMQYFLST
GNLVSQSGLDLQQVSGYTVVAEKINFYRFISHFRMVHRGSFFAQLKTTTV
RKLLPESWGFLCPVHTPDGSPCGLLNHFAHKCRISTQQSDVSRIPSILYS
LGVAPASHTFAAGPSLCCVQIDGKIIGWVSHEQGKIIADTLRYWKVEGKT
PGLPIDLEIGYVPPSTRGQYPGLYLFGGHSRMLRPVRYLPLDKEDIVGPF
EQVYMNIAVTPQEIQNNVHTHVEFTPTNILSILANLTPFSDFNQSPRNMY
QCQMGKQTMGTPGVALCHRSDNKLYRLQTGQTPIVKANLYDDYGMDNFPN
GFNAVVAVISYTGYDMDDAMIINKSADERGFGYGTMYKTEKVDLALNRNR
GDPITQHFGFGNDEWPKEWLEKLDEDGLPYIGTYVEEGDPICAYFDDTLN
KTKIKTYHSSEPAYIEEVNLIGDESNKFQELQTVSIKYRIRRTPQIGDKF
SSRHGQKGVCSRKWPTIDMPFSETGIQPDIIINPHAFPSRMTIGMFVESL
AGKAGALHGIAQDSTPWIFNEDDTPADYFGEQLAKAGYNYHGNEPMYSGA
TGEELRADIYVGVVYYQRLRHMVNDKFQVRSTGPVNSLTMQPVKGRKRHG
GIRVGEMERDALIGHGTSFLLQDRLLNSSDYTQASVCRECGSILTTQQSV
PRIGSISTVCCRRCSMRFEDAKKLIFIDDSQIWEDGQGNKFVGGNETTTV
AIPFVLKYLDSELSAMGIRLRYNVEPK
3D structure
PDB5n61 Structural Basis of RNA Polymerase I Transcription Initiation.
ChainB
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna B K916 K924 H1038 K899 K907 H1021
BS02 dna B V113 K460 Y463 N893 F895 K1061 R1063 R1070 M1074 V96 K443 Y446 N876 F878 K1044 R1046 R1053 M1057
BS03 dna B N816 R817 N799 R800
BS04 ZN B C1104 C1107 C1128 C1131 C1087 C1090 C1111 C1114
Gene Ontology
Molecular Function
GO:0001054 RNA polymerase I activity
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006360 transcription by RNA polymerase I
GO:0006361 transcription initiation at RNA polymerase I promoter
GO:0006362 transcription elongation by RNA polymerase I
GO:0006363 termination of RNA polymerase I transcription
GO:0042254 ribosome biogenesis
GO:0042790 nucleolar large rRNA transcription by RNA polymerase I
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005736 RNA polymerase I complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5n61, PDBe:5n61, PDBj:5n61
PDBsum5n61
PubMed28340337
UniProtP22138|RPA2_YEAST DNA-directed RNA polymerase I subunit RPA135 (Gene Name=RPA135)

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