Structure of PDB 5mdh Chain B

Receptor sequence
>5mdhB (length=333) Species: 9823 (Sus scrofa) [Search protein sequence]
SEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLD
GVLMELQDCALPLLKDVIATDKEEIAFKDLDVAILVGSMPRRDGMERKDL
LKANVKIFKCQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKEN
FSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVK
LQAKEVGVYEAVKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAIC
DHVRDIWFGTPEGEFVSMGIISDGNSYGVPDDLLYSFPVTIKDKTWKIVE
GLPINDFSREKMDLTAKELAEEKETAFEFLSSA
3D structure
PDB5mdh Structural basis of substrate specificity in malate dehydrogenases: crystal structure of a ternary complex of porcine cytoplasmic malate dehydrogenase, alpha-ketomalonate and tetrahydoNAD.
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D158 H186
Catalytic site (residue number reindexed from 1) D158 H186
Enzyme Commision number 1.1.1.37: malate dehydrogenase.
1.1.1.96: diiodophenylpyruvate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAD B G10 G13 Q14 I15 D41 I42 V86 S88 I107 V128 G129 N130 L154 L157 H186 S240 G10 G13 Q14 I15 D41 I42 V86 S88 I107 V128 G129 N130 L154 L157 H186 S240
BS02 MAK B R91 R97 L157 R161 H186 G230 R91 R97 L157 R161 H186 G230
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0016491 oxidoreductase activity
GO:0016615 malate dehydrogenase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0030060 L-malate dehydrogenase (NAD+) activity
GO:0047995 hydroxyphenylpyruvate reductase activity
GO:0051287 NAD binding
Biological Process
GO:0006099 tricarboxylic acid cycle
GO:0006107 oxaloacetate metabolic process
GO:0006108 malate metabolic process
GO:0006734 NADH metabolic process
GO:0019752 carboxylic acid metabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5mdh, PDBe:5mdh, PDBj:5mdh
PDBsum5mdh
PubMed10075524
UniProtP11708|MDHC_PIG Malate dehydrogenase, cytoplasmic (Gene Name=MDH1)

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