Structure of PDB 5m8w Chain B

Receptor sequence

>5m8wB (length=419) Species: 66821 (Sulfurospirillum multivorans)

AAEIRQQFAMTAGSPIIVNDKLERYAEVRTAFTHPTSFFKPNYKGEVKPW
FLSAYDEKVRQIENGENGPKMKAKNVGEARAGRALEAAGWTLDINYGNIY
PNRFFMLWSGETMTNTQLWAPVGLDRRPPDTTDPVELTNYVKFAARMAGA
DLVGVARLNRNWVYSEAVTIPADVPYEQSLHKEIEKPIVFKDVPLPIETD
DELIIPNTCENVIVAGIAMNREMMQTAPNSMACATTAFCYSRMCMFDMWL
CQFIRYMGYYAIPSCNGVGQSVAFAVEAGLGQASRMGACITPEFGPNVRL
TKVFTNMPLVPDKPIDFGVTEFCETCKKCARECPSKAITEGPRTFEGRSI
HNQSGKLQWQNDYNKCLGYWPESGGYCGVCVAVCPFTKNITEVWDGKINT
YGLDADHFRDTVSFRKDRV

3D structure

• PDB: 5m8w Cobamide-mediated enzymatic reductive dehalogenation via long-range electron transfer.
• Chain: B
• Resolution: 2.279 Å

Enzymatic activity

• Enzyme Commision number: 1.21.99.5: tetrachloroethene reductive dehalogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	R291 M292 I296 F328 C329 C332 C335 C390 P391 F392	R285 M286 I290 F322 C323 C326 C329 C384 P385 F386
BS02	SF4	B	C339 P340 I344 C372 C383 C386	C333 P334 I338 C366 C377 C380
BS03	BVQ	B	Y31 T36 A37 F38 Y170 Y246 M249 N272 Q276 S277 V278 A289 M292 G293 A294 C295 V304 R305 L306 H357 N358 Q359 K362 Q364 Y369 C372 W376 Y382 C386	Y25 T30 A31 F32 Y164 Y240 M243 N266 Q270 S271 V272 A283 M286 G287 A288 C289 V298 R299 L300 H351 N352 Q353 K356 Q358 Y363 C366 W370 Y376 C380
BS04	4CH	B	F38 W96 Y102 Y246 W376 Y382	F32 W90 Y96 Y240 W370 Y376

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:5m8w, PDBe:5m8w, PDBj:5m8w
• PDBsum: 5m8w
• PubMed: 28671181
• UniProt: O68252|PCEA_SULMU Tetrachloroethene reductive dehalogenase (Gene Name=pceA)