Structure of PDB 5lgv Chain B

Receptor sequence

>5lgvB (length=649) Species: 100226 (Streptomyces coelicolor A3(2))

PTVVGRIPVLDVRPVVQRGRRPAKAVTGESFEVSATVFREGHDAVGANVV
LRDPRGRPGPWTPMRELAPGTDRWGATVTAGETGTWSYTVEAWGDPVTTW
RHHARIKIPAGLDTDLVLEEGARLYERAAADVPGREDRRELLAAVDALRD
ESRPAASRLAAALTPQVDAVLARHPLRDLVTSSDPLPLLVERERALYGAW
YEFFPRSEGTPHTPHGTFRTAARRLPAIAAMGFDVVYLPPIHPIGTTHRK
GRNNTLSATGDDVGVPWAIGSPEGGHDSIHPALGTLDDFDHFVTEAGKLG
LEIALDFALQCSPDHPWVHKHPEWFHHRPDGTIAHAENPPKKYQDIYPIA
FDADPDGLATETVRILRHWMDHGVRIFRVDNPHTKPVAFWERVIADINGT
DPDVIFLAAAFTRPAMMATLAQIGFQQSYTYFTWRNTKQELTEYLTELSG
EAASYMRPNFFANTPDILHAYLQHGGRPAFEVRAVLAATLSPTWGIYSGY
ELCENTPLREGSEEYLDSEKYQLKPRDWTRAAREGTTIAPLVTRLNTIRR
ENPALRQLRDLHFHPTDKEEVIAYSKRQGSNTVLVVVNLDPRHTQEATVS
LDMPQLGLDWHESVPVRDELTGETYHWGRANYVRLEPGRTPAHVCTVLR

3D structure

• PDB: 5lgv Ligand-bound structures and site-directed mutagenesis identify the acceptor and secondary binding sites of Streptomyces coelicolor maltosyltransferase GlgE.
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.4.99.16: starch synthase (maltosyl-transferring).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	B	R427 Y445 R449	R413 Y431 R435
BS02	GLC	B	F425 W448	F411 W434
BS03	GLC	B	K355 F425	K341 F411
BS04	GLC	B	K355 Y357 N395 H397	K341 Y343 N381 H383
BS05	GLC	B	Y357 D394 N395 D480	Y343 D380 N381 D466
BS06	GLC	B	K264 V279 W281 A282 Y357 D359 K534 Y535	K250 V265 W267 A268 Y343 D345 K520 Y521
BS07	GLC	B	D581 E610	D567 E596
BS08	GLC	B	Y639 Y646 V647 R648 H657	Y625 Y632 V633 R634 H643

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0004556 alpha-amylase activity
• GO:0016757 glycosyltransferase activity
• GO:0016758 hexosyltransferase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0009313 oligosaccharide catabolic process
• GO:0030979 alpha-glucan biosynthetic process

External links

• PDB: RCSB:5lgv, PDBe:5lgv, PDBj:5lgv
• PDBsum: 5lgv
• PubMed: 27531751
• UniProt: Q9L1K2|GLGE1_STRCO Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1 (Gene Name=glgE1)