Structure of PDB 5la3 Chain B

Receptor sequence

>5la3B (length=575) Species: 1501 (Clostridium pasteurianum) [Search protein sequence]

TIIINGVQFNTDEDTTILKFARDNNIDISALCFLNNCNNDINKCEICTVE
VEGTGLVTACDTLIEDGMIINTNSDAVNEKIKSRISQLLDIHEFKCGPCN
RRENCEFLKLVIKYKARASKPFLPKDKTEYVDERSKSLTVDRTKCLLCGR
CVNACGKNTETYAMKFLNKNGKTIIGAEDEKCFDDTNCLLCGQCIIACPV
AALSEKSHMDRVKNALNAPEKHVIVAMAPSVRASIGELFNMGFGVDVTGK
IYTALRQLGFDKIFDINFGADMTIMEAATELVQRIENNGPFPMFTSCCPG
WVRQAENYYPELLNNLSSAKSPQQIFGTASKTYYPSISGLDPKNVFTVTV
MPCTSKKFEADRPQMEKDGLRDIDAVITTRELAKMIKDAKIPFAKLEDSE
ADPAMGEYSGAGAIFGATGGVMEAALRSAKDFAENAELEDIEYKQVRGLN
GIKEAEVEINNNKYNVAVINGASNLFKFMKSGMINEKQYHFIEVMACHGG
CVNGGGQPHVNPKDLEKVDIKKVRASVLYNQDEHLSKRKSHENTALVKMY
QNYFGKPGEGRAHEILHFKYKKSAW

3D structure

PDB

5la3 Accumulating the hydride state in the catalytic cycle of [FeFe]-hydrogenases.

Chain

Resolution

2.29 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	A279 E282 C299 S319 K358 E361 Q366 C503
Catalytic site (residue number reindexed from 1)	A277 E280 C297 S317 K356 E359 Q364 C501
Enzyme Commision number	1.12.7.2: ferredoxin hydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	402	B	A230 P231 S232 C299 P324 Q325 P354 K358 F417 M497 C503	A228 P229 S230 C297 P322 Q323 P352 K356 F415 M495 C501
BS02	SF4	B	C300 C355 A498 C499 C503	C298 C353 A496 C497 C501
BS03	SF4	B	C157 M166 C190 L191 L192 C193 G194 C196	C155 M164 C188 L189 L190 C191 G192 C194
BS04	SF4	B	C147 L148 L149 C150 G151 C153 I177 C200 P201 A204 L205	C145 L146 L147 C148 G149 C151 I175 C198 P199 A202 L203
BS05	SF4	B	H94 F96 K97 C98 C101 R104 C107 V202	H92 F94 K95 C96 C99 R102 C105 V200
BS06	FES	B	A32 C34 F35 C46 E47 C49 C62	A30 C32 F33 C44 E45 C47 C60

Gene Ontology

	Molecular Function
GO:0005506	iron ion binding
GO:0008901	ferredoxin hydrogenase activity
GO:0016491	oxidoreductase activity
GO:0046872	metal ion binding
GO:0051536	iron-sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

View graph for
Molecular Function

External links

PDB	RCSB:5la3, PDBe:5la3, PDBj:5la3
PDBsum	5la3
PubMed	28722011
UniProt	P29166\|PHF1_CLOPA Iron hydrogenase 1

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