Structure of PDB 5l44 Chain B

Receptor sequence

>5l44B (length=662) Species: 592016 (Astrosporangium hypotensionis K-26)

ENPFFAPSALPYGLPPFAEIREEHYVPAFERGMAEQLAEVEAIAGDTEAP
TFDNTVAALERSGQVLTRVSAVFFNQSSSDTNPTVQEIQKQIIPKLTQHG
DAIHLNRPLFARIKQISPDGLDAEQAWLLERYVTDFVRAGAELGAGDQER
LKALNEELSTLSTRFEQNLLAHTNASAVIVDDVAQLDGLSDDSVKAAAET
AKSRGLPGKYVIPLVLPTGQPGLAELTDRALRERIHRASIQRGVPDNEEL
IVRIATLRAERAKLLGYPTHAAYVVADQTAPTTEAVTEMLGKLTPPAVAN
AHREADELREQAGHDLEPWDWSFYAEKVLKERYAIDGRQMRPYFELDRVL
RDGVFHAATLLYGITFTERPDLVGYHPDVRVFEVFNEDGSQLGLFLGDYY
ARPSKRGGAWMNSLVKQSTLEGTRPVVVNNLNIAKPPAGEPTLMTFEEVN
TMFHEFGHALHGLFSEVHYPRFSGTAVPRDFVEYPSQVNEMWAVWPSVLA
NYARHWQTGDPMPKDLLDRMLKSQKYNQGYKTVEYLAATLLDWSWHTFQT
PPENALTFEHEALTTAGVDLKLVPPRYRSTYFAHIWSSGYSAGYYSYIWS
EVLDADTVDWFHENGGLLRENGDTFRQKLLSKGGSVDPMTAFQSFRGRTP
RIEPLLDRRGLL

3D structure

• PDB: 5l44 Crystal structure of a peptidyl-dipeptidase K-26-DCP from Actinomycete in complex with its natural inhibitor.
• Chain: B
• Resolution: 1.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H463 E464 H467 Y606
• Catalytic site (residue number reindexed from 1): H454 E455 H458 Y597

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	H463 H467 E492	H454 H458 E483
BS02	MG	B	D524 D527	D515 D518
BS03	K26	B	R415 A418 W419 M420 H463 E464 H467 T484 R488 E492 H593 S597 Y599 Y606	R406 A409 W410 M411 H454 E455 H458 T475 R479 E483 H584 S588 Y590 Y597

Gene Ontology

Molecular Function

• GO:0004180 carboxypeptidase activity
• GO:0004222 metalloendopeptidase activity
• GO:0008233 peptidase activity
• GO:0008237 metallopeptidase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005829 cytosol
• GO:0030288 outer membrane-bounded periplasmic space

External links

• PDB: RCSB:5l44, PDBe:5l44, PDBj:5l44
• PDBsum: 5l44
• PubMed: 27754586
• UniProt: A0A1L1QK30