Structure of PDB 5kgt Chain B

Receptor sequence

>5kgtB (length=429) Species: 233413 (Mycobacterium tuberculosis variant bovis AF2122/97)

GLTPEQIIAVDGAHLWHPYSSIGREAVSPVVAVAAHGAWLTLIRDGQPIE
VLDAMSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKL
LVDITPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGG
YHGDTFLAMSICDPHGGMHSLWTDVLAAQVFAPQVPRDYDPAYSAAFEAQ
LAQHAGELAAVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEI
ATGFGRTGALFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTIS
AGAAGALMHGPTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGL
DTARALPAVTDVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLV
YAMPPYICTPAEITQITSAMVEVARLVGS

3D structure

• PDB: 5kgt Structure-Based Optimization of Pyridoxal 5'-Phosphate-Dependent Transaminase Enzyme (BioA) Inhibitors that Target Biotin Biosynthesis in Mycobacterium tuberculosis.
• Chain: B
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y25 Y157 E220 D254 A257 K283 Y407
• Catalytic site (residue number reindexed from 1): Y19 Y151 E214 D248 A251 K277 Y401
• Enzyme Commision number: 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	B	P317 T318	P311 T312
BS02	6SQ	B	P24 Y25 W64 Y157 D169 G172 G173	P18 Y19 W58 Y151 D163 G166 G167
BS03	PLP	B	G124 S125 Y157 H158 G159 E220 D254 I256 K283	G118 S119 Y151 H152 G153 E214 D248 I250 K277

Gene Ontology

Molecular Function

• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5kgt, PDBe:5kgt, PDBj:5kgt
• PDBsum: 5kgt
• PubMed: 28594172
• UniProt: P0A4X7|BIOA_MYCBO Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)