Structure of PDB 5k85 Chain B

Receptor sequence

>5k85B (length=647) Species: 235443 (Cryptococcus neoformans var. grubii H99)

HHVHPLPDSVPESEDLFAPPPRMQGKEGRPKPHIGPNYESYVKEWAKTVG
PNSDEWWAAKARETLDWYDDFKTVRAGGFEHGDVQWFPEGTLNAAYNCLD
RHYYKNPKKTAIIYEADEPSESREVSYEELMQETCRVANVLKSYGVKKGD
AVSIYLPMTWQAAAAFLACARIGAIHSAVFAGFSAESLRDRVNDCECKVL
ITTDEGRRGGKTIATKQIVDAALQQCPLVENVLVLRRTGNKVPMTEGRDK
WWDEECAKMPAYCPCERMASEDPLFILYTSGKPKGVVHSTAGYLLGTALT
LKYVFDAHPDDRFACMADIGWITGHSYIIYGPLANGITTAVFESTPVYPT
PSRYWDFVDKWKATQLYTAPTAIRLLRRMGEDHVKNHDLSSLRVLGSVGE
PINPEAWHWYNDFAGKNQCAIVDTYWMTETGSISIAPLPGAISTKPGSAT
FPFFGMDVDIIDPQTGQVLEGNDVEGVLVARRPWPSIARTVYRDHKRYLE
TYMKPYPGYFFFGDGAARDYDGYMWIKGRVDDVINVSGHRLSTAEVESAL
ILHKGVAETAVVGCADDLTGQAVYAFVTMKPEFDLKEADLSKELAIQVRK
VIGPFAAPKKIYLVSDLPKTRSGKIMRRVLRKIVAPQIVEEVKQKVT

3D structure

• PDB: 5k85 CRYSTAL STRUCTURE OF ACETYL-COA SYNTHETASE IN COMPLEX WITH ADENOSINE-5'-PROPYLPHOSPHATE AND COENZYME A FROM CRYPTOCOCCUS NEOFORMANS H99
• Chain: B
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T289 T441 E442 N548 R553 K640
• Catalytic site (residue number reindexed from 1): T279 T428 E429 N535 R540 K624
• Enzyme Commision number: 6.2.1.1: acetate--CoA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PRX	B	W334 V411 G412 E413 P414 T437 Y438 W439 M440 T441 D527 I539 R542	W321 V398 G399 E400 P401 T424 Y425 W426 M427 T428 D514 I526 R529

Gene Ontology

Molecular Function

• GO:0003987 acetate-CoA ligase activity
• GO:0005524 ATP binding
• GO:0016208 AMP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006085 acetyl-CoA biosynthetic process
• GO:0019427 acetyl-CoA biosynthetic process from acetate

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5k85, PDBe:5k85, PDBj:5k85
• PDBsum: 5k85
• UniProt: J9VFT1