Structure of PDB 5k0s Chain B

Receptor sequence
>5k0sB (length=485) Species: 204722 (Brucella suis 1330) [Search protein sequence]
REKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDE
HGIKMLQSARKEGITPRDLADRNTSAFRRMAEVLNSSNDDYIRTSEERHY
KASQAIWQAMVANGDIYKGGYAGWYSVRDEAYYGEEERYGPTPVEWVEEE
SYFFRLSAYQDKLLDLYENNPGFIMPAERRNEIVSFVKSGLKDLSISRTT
FDWGIPVPGDEKHVMYVWVDALTNYITALGYPDTTDERWAYWPANAHIIG
KDISRFHAVYWPAFLMSAQLPLPKRVFAHGFLFIDPFELVERYGLDQLRY
FLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCEGKV
PQPGAFSEADKAILDQADAALETARKAMDDQALHLALGAIFAVVAEANRY
FAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPFIPQSAEKLLDIL
AVPADKRQFADVLASPLAGGTDLPAPQPVFPRYVE
3D structure
PDB5k0s Brucella melitensis Methionyl-tRNA-Synthetase (MetRS), a Potential Drug Target for Brucellosis.
ChainB
Resolution2.45 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) I12 H20 H23 D51 S128 D131 G150 W230 I265
Catalytic site (residue number reindexed from 1) I10 H18 H21 D49 S126 D129 G140 W218 I253
Enzyme Commision number 6.1.1.10: methionine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 0OU B A11 I12 Y14 D51 H53 G54 F213 M227 Y228 V229 D232 A233 F268 H269 A9 I10 Y12 D49 H51 G52 F201 M215 Y216 V217 D220 A221 F256 H257 MOAD: ic50=0.006uM
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004825 methionine-tRNA ligase activity
GO:0005524 ATP binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006431 methionyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm

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Biological Process
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Cellular Component
External links
PDB RCSB:5k0s, PDBe:5k0s, PDBj:5k0s
PDBsum5k0s
PubMed27500735
UniProtP59078|SYM_BRUSU Methionine--tRNA ligase (Gene Name=metG)

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