Structure of PDB 5jpi Chain B

Receptor sequence

>5jpiB (length=491) Species: 5807 (Cryptosporidium parvum)

ESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGSLH
LTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAWKN
ETIEDYWVCLNDAMTWRNPNKDKICGPNLIVDDGGDATLILHEGVKAEIE
YEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGMLKD
LYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQSL
LHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPICA
LQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIANI
GHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLVNL
GCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKELD
EYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5jpi 2.15 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with D-Eritadenine and NAD.
• Chain: B
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H50 S73 S78 D133 E207 N232 K237 D241 N242 C246 H352 H406 S414 Q418
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	B	K489 Y493	K485 Y489
BS02	NAD	B	T212 T213 T214 N246 G275 G277 E278 V279 T297 E298 I299 T331 N333 I354 G355 H356 N403	T208 T209 T210 N242 G271 G273 E274 V275 T293 E294 I295 T327 N329 I350 G351 H352 N399
BS03	DEA	B	H53 T55 E57 E211 T212 K241 D245 L404 G409 H410 M415	H50 T52 E54 E207 T208 K237 D241 L400 G405 H406 M411

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5jpi, PDBe:5jpi, PDBj:5jpi
• PDBsum: 5jpi
• UniProt: Q5CPH1