Structure of PDB 5jh9 Chain B

Receptor sequence

>5jh9B (length=477) Species: 559292 (Saccharomyces cerevisiae S288C)

EEKEKKENENSWCILEHNYEDIAQEFIDFIYKNPTTYHVVSFFAELLDKH
NFKYLSEKSNWQDSIGEDGGKFYTIRNGTNLSAFILGKNWRAEKGVGVIG
SHVDALTVKLKPVSFKDTAEGYGRIAVAPYGGTLNELWLDRDLGIGGRLL
YKKKGTNEIKSALVDSTPLPVCRIPSLAPHFGKPAEGPFDKEDQTIPVIG
FPTPEPPTDDEKKSPLFGKHCIHLLRYVAKLAGVEVSELIQMDLDLFDVQ
KGTIGGIGKHFLFAPRLDDRLCSFAAMIALICYAKDVNTEESDLFSTVTL
YDNEEIGSLTRQGAKGGLLESVVERSSSAFTKKPVDLHTVWANSIILSAD
VNHLYNPNFPEVYLKNHFPVPNVGITLSLDPNGHMATDVVGTALVEELAR
RNGDKVQYFQIKNNSRSGGTIGPSLASQTGARTIDLGIAQLSMHSIRAAT
GSKDVGLGVKFFNGFFKHWRSVYDEFG

3D structure

• PDB: 5jh9 Structural Basis for Receptor-Mediated Selective Autophagy of Aminopeptidase I Aggregates
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 3.4.11.22: aminopeptidase I.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D303 D385	D268 D350
BS02	ZN	B	D303 E340 H479	D268 E305 H444

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0005515 protein binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0015031 protein transport
• GO:0032258 cytoplasm to vacuole targeting by the Cvt pathway

Cellular Component

• GO:0000324 fungal-type vacuole
• GO:0005737 cytoplasm
• GO:0005773 vacuole
• GO:0034270 Cvt complex

External links

• PDB: RCSB:5jh9, PDBe:5jh9, PDBj:5jh9
• PDBsum: 5jh9
• PubMed: 27320913
• UniProt: P14904|AMPL_YEAST Vacuolar aminopeptidase 1 (Gene Name=APE1)