Structure of PDB 5izl Chain B

Receptor sequence

>5izlB (length=498) Species: 9606 (Homo sapiens)

AGRRVNVNVGVLGHIDSGKTALARALSTTASRGITLDLGFSCFSVPLPAR
LRSSLPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQT
QSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTK
FRGAPIIPVAAKPGGPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSV
DHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPIT
SAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGP
LQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQ
YLSKGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGI
LLHGLEDRNYADSFLPRLKVYKLKHKRAMDDYSVINIQLFVGLKVHLSTG
ELGIIDSGKFKIHIPGGLSPESKKILHVVLSLTFKRYVFDTHKRMVQS

3D structure

• PDB: 5izl Crystal structures of the human elongation factor eEFSec suggest a non-canonical mechanism for selenocysteine incorporation.
• Chain: B
• Resolution: 2.72 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D17 K20 T21 T48 H96
• Catalytic site (residue number reindexed from 1): D16 K19 T20 T35 H71
• Enzyme Commision number: 3.6.5.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	T21 T48	T20 T35

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0035368 selenocysteine insertion sequence binding
• GO:0043021 ribonucleoprotein complex binding

Biological Process

• GO:0001514 selenocysteine incorporation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5izl, PDBe:5izl, PDBj:5izl
• PDBsum: 5izl
• PubMed: 27708257
• UniProt: P57772|SELB_HUMAN Selenocysteine-specific elongation factor (Gene Name=EEFSEC)