Structure of PDB 5iq1 Chain B

Receptor sequence

>5iq1B (length=445) Species: 89187 (Roseovarius nubinhibens ISM)

TKRVAVIGAGPSGLAQLRAFQSAADQGAEIPEIVCFEKQANWGGLWNYTW
RTGLDENGEPVHCSMYRYLWSNGPKEGLEFADYSFEEHFGKQIASYPPRA
VLFDYIEGRVHKADVRKWIRFNSPVRWVSYDAETAKFTVTAHNHETDSTY
SAAFDHVICASGHFSTPNVPFYEGFDTFNGRIVHAHDFRDAREFEGKDVL
VMGASSSAEDIGSQCWKYGAKSITSCYRSAPMGYAWPDNWEEKPALEKLT
GKTAHFADGSTRDVDAIILCTGYKHFFSFLPDDLRLKTANRLATADLYKG
VAYVHNPAMFYLGMQDQWFTFNMFDAQAWWVRDAILGRITLPKDKAAMLA
DVAERETREEASDDVKYAIRYQADYVKELVAETDYPSFDIDGACDAFFEW
KKHKAKDIMAFRDNSYKSVITGTMAPVHHTPWKEALDDSMEAYLQ

3D structure

• PDB: 5iq1 Structural mechanism for bacterial oxidation of oceanic trimethylamine into trimethylamine N-oxide
• Chain: B
• Resolution: 1.75 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.148: trimethylamine monooxygenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	B	Y67 W71 N73 F165 N169 P171 Y173 S206 S207 S208 D211 R229 S230 C271 T272 G273	Y66 W70 N72 F164 N168 P170 Y172 S205 S206 S207 D210 R228 S229 C270 T271 G272
BS02	FAD	B	G9 G11 P12 S13 F37 E38 K39 Q40 G45 L46 W47 H63 M66 S72 N73 P125 V126 A161 S162 G163 F165 F280 Q318 T321 F325	G8 G10 P11 S12 F36 E37 K38 Q39 G44 L45 W46 H62 M65 S71 N72 P124 V125 A160 S161 G162 F164 F279 Q317 T320 F324

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0004499 N,N-dimethylaniline monooxygenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

External links

• PDB: RCSB:5iq1, PDBe:5iq1, PDBj:5iq1
• PDBsum: 5iq1
• PubMed: 27997715
• UniProt: A3SLM3|TMM_ROSNI Trimethylamine monooxygenase (Gene Name=tmm)