Structure of PDB 5imq Chain B

Receptor sequence

>5imqB (length=591) Species: 300852 (Thermus thermophilus HB8)

MDLSRIRNFSIIAHVDHGKSTLADRILELTHAVSDREMREQFLDSLELER
ERGITIKASAVRVTYRAKDGEEYVFHLIDTPGHVDFTYEVSRALAAVEGV
LLVVDASQGVEAETLAKFYMALEHGHVIIPVINKIDLPNARPLEVALEVE
EVLGLPADEAIFASGKTGEGVEEILEAIVQRIPPPKGDPEAPLKALIFDS
VYDAYQGVIPYLRLFEGRVRPGDRIRIYSTGKEFTVDKVGVFTPQGLVAT
EALEAGEVGWLVAAIRDIHDVQVGDTITLADRPTPSPYPGFRPAKPVVFA
GLYPVGKLRDALEKLKLNDAALTFEPESSTALGFGFRCGFLGLLHAEIVQ
ERLEREFGLSLIATAPSVVYKVRLKSGEEVEVHNPADLPDPTRIEEILEP
YVKLTIFTPEEYVGSLMQLLQEKRGRLVNMNYLPGAQKRVELVYEAPFAE
ILYDFHDRLKSVSRGYASMDYEQAGYRPGDLVKVNVLVHGEVVDALTFIA
HREKAYTMARAIVDKLAEVIPRQLFEVPIQAAIGGKIIARATVKALRKDV
LAKCYGGDVTRKKKLLEKQKEGKKRLKAIGKVEVPQEAFLA

3D structure

• PDB: 5imq Structure of the GTP Form of Elongation Factor 4 (EF4) Bound to the Ribosome
• Chain: B
• Resolution: 3.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D19 K22 S23 T58 H86
• Catalytic site (residue number reindexed from 1): D16 K19 S20 T55 H83
• Enzyme Commision number: 3.6.5.n1: elongation factor 4.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	R569 K572	R561 K564
BS02	rna	B	Y208 R269	Y205 R266
BS03	rna	B	H17 E114 D465 R472 R530 Q531 L532 L554 R555 K556 K561 C562 G564 K576 Q577 K581	H14 E111 D457 R464 R522 Q523 L524 L546 R547 K548 K553 C554 G556 K568 Q569 K573

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0043022 ribosome binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0045727 positive regulation of translation

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:5imq, PDBe:5imq, PDBj:5imq
• PDBsum: 5imq
• PubMed: 27137929
• UniProt: Q5SKA7|LEPA_THET8 Elongation factor 4 (Gene Name=lepA)