Structure of PDB 5hxw Chain B

Receptor sequence

>5hxwB (length=432) Species: 585 (Proteus vulgaris)

RHGFVEGTEGALPKQADVVVVGAGILGIMTAINLVERGLSVVIVEKGNIA
GEQSSRFYGQAISYKMPDETFLLHHLGKHRWREMNAKVGIDTTYRTQGRV
EVPLDEEDLVNVRKWIDERSKNVGSDIPFKTRIIEGAELNQRLRGATTDW
KIAGFEEDSGSFDPEVATFVMAEYAKKMGVRIYTQCAARGLETQAGVISD
VVTEKGAIKTSQVVVAGGVWSRLFMQNLNVDVPTLPAYQSQQLISGSPTA
PGGNVALPGGIFFREQADGTYATSPRVIVAPVVKESFTYGYKYLPLLALP
DFPVHISLNEQLINSFMQSTHWNLDEVSPFEQFRNMTALPDLPELNASLE
KLKAEFPAFKESKLIDQWSGAMAIAPDENPIISEVKEYPGLVINTATGWG
MTESPVSAELTADLLLGKKPVLDPKPFSLYRF

3D structure

• PDB: 5hxw Crystal structure of a membrane-bound l-amino acid deaminase from Proteus vulgaris
• Chain: B
• Resolution: 2.63 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): F96 Q99 A100 Q278 V322 F326
• Catalytic site (residue number reindexed from 1): F57 Q60 A61 Q239 V283 F287
• Enzyme Commision number: 1.4.3.2: L-amino-acid oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	V60 G61 G63 I64 L65 V83 E84 K85 E91 Q92 S93 F96 Y97 G98 Q99 A226 A227 G256 G257 W259 Q278 Q280 F369 G409 M411 T436 G437 W438 G439 M440 T441	V21 G22 G24 I25 L26 V44 E45 K46 E52 Q53 S54 F57 Y58 G59 Q60 A187 A188 G217 G218 W220 Q239 Q241 F330 G370 M372 T397 G398 W399 G400 M401 T402
BS02	16A	B	N348 I352 F355	N309 I313 F316
BS03	16A	B	F326 L347	F287 L308
BS04	16A	B	M356 F372	M317 F333
BS05	16A	B	G329 Y332 F341 E417	G290 Y293 F302 E378

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5hxw, PDBe:5hxw, PDBj:5hxw
• PDBsum: 5hxw
• PubMed: 27422658
• UniProt: Q9LCB2