Structure of PDB 5hj5 Chain B

Receptor sequence

>5hj5B (length=267) Species: 243277 (Vibrio cholerae O1 biovar El Tor str. N16961)

MRLIPLKAAAQVGKWAAAHIVKRINEFQPTAERPFVLGLPTGGTPLATYK
ALIEMHKAGEVSFKHVVTFNMDEYVGLAADHPESYRSFMYNNFFNHIDIQ
EENINLLNGNTDDHEAECKRYEDKIKSYGKINLFMGGVGNDGHIAFNEPA
SSLSSRTRIKTLTEDTRIANSRFFDGDINQVPKYALTIGVGTLLDAQEIM
ILVTGHNKALALQAAVEGSVNHLWTVSALQLHPKAVIVCDEPSTQELKVK
TVKYFTELEAKNIVGFR

3D structure

• PDB: 5hj5 Crystal structure of tertiary complex of glucosamine-6-phosphate deaminase from Vibrio cholerae with BETA-D-GLUCOSE-6-PHOSPHATE and FRUCTOSE -6-PHOSPHATE
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D72 D141 H143 E148
• Catalytic site (residue number reindexed from 1): D72 D141 H143 E148
• Enzyme Commision number: 3.5.99.6: glucosamine-6-phosphate deaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F6R	B	T41 G42 G43 T44 D72 G137 V138 H143 A145 R172 K208	T41 G42 G43 T44 D72 G137 V138 H143 A145 R172 K208
BS02	BG6	B	M1 R2	M1 R2

Gene Ontology

Molecular Function

• GO:0004342 glucosamine-6-phosphate deaminase activity
• GO:0016787 hydrolase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006043 glucosamine catabolic process
• GO:0006044 N-acetylglucosamine metabolic process
• GO:0006046 N-acetylglucosamine catabolic process
• GO:0019262 N-acetylneuraminate catabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5hj5, PDBe:5hj5, PDBj:5hj5
• PDBsum: 5hj5
• UniProt: Q9KKS5|NAGB_VIBCH Glucosamine-6-phosphate deaminase (Gene Name=nagB)