Structure of PDB 5gkf Chain B

Receptor sequence
>5gkfB (length=239) Species: 69014 (Thermococcus kodakarensis KOD1) [Search protein sequence]
SKDKVTVITSPSTEELVSLVNSALLEEAMLTIFARCKVHYDGRAKSELGS
GDRVIIVKPDGSFLIHQSKKREPVNWQPPGSRVRLELRENPVLVSIRRKP
RETLEVELEEVYMVSVFRAEDYEELALTGSEAEMAELIFENPEVIEPGFK
PLFREKAIGTGIVAVLGRDSDGNIVVLELKRRRAELHAVRQLKSYVEILR
EEYGDKVRGILVAPSLTSGAKRLLEKEGLEFRKLEPPKR
3D structure
PDB5gkf Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease
ChainB
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B K71 R72 S131 E132 G162 I163 E179 K181 R182 R183 Q192 Y196 K70 R71 S130 E131 G161 I162 E178 K180 R181 R182 Q191 Y195 PDBbind-CN: Kd=2.8nM
BS02 dna B Y41 R44 N76 W77 P80 R191 T218 G220 Y40 R43 N75 W76 P79 R190 T217 G219 PDBbind-CN: Kd=2.8nM
Gene Ontology
Molecular Function
GO:0000014 single-stranded DNA endodeoxyribonuclease activity
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
Biological Process
GO:0006259 DNA metabolic process
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:5gkf, PDBe:5gkf, PDBj:5gkf
PDBsum5gkf
PubMed27773688
UniProtQ5JER9|NUCS_THEKO Endonuclease NucS (Gene Name=nucS)

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