Structure of PDB 5fw1 Chain B

Receptor sequence

>5fw1B (length=1304) Species: 1314 (Streptococcus pyogenes)

KYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLF
DSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRL
IYLALAHMIKFRGHFLIEGDLNPDSVDKLFIQLVQTYNQLFEENPINASG
VDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSN
FDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDI
LRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQS
KNGYAGYIDGGASQEEFYKFIKPILEKMDTEELLVKLNREDLLRKQRTFD
NGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLA
RGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEK
VLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTN
RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDF
LDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRY
TGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKE
DIQKSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMA
RNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYV
DQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEE
VVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVET
RQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYK
VREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDGKATAKYFFYSN
IMNFFKTEIKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE
VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKV
EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLP
KYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHEQKQLFVE
QHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
HLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLDATLIHQSITGLYETRI
DLSQ

3D structure

• PDB: 5fw1 Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.
• Chain: B
• Resolution: 2.499 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	K33 L35 G56 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 Y81 L101 E102 S104 F105 V107 K111 R115 H116 I122 V126 H129 P133 T134 I135 K163 F164 R165 G166 Y325 H328 H329 K336 R340 F351 F352 Y359 A360 I363 K401 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 K510 Y515 T519 N588 R661 M694 H721 K735 Q739 K742 T893 K1097 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 R1122 K1123 K1124 W1126 K1130 Y1131 M1169 H1349 Q1350 Y1356 T1358	K30 L32 G53 E54 T55 A56 T59 R60 K62 R63 R66 R67 R68 Y69 R71 R72 K73 N74 Y78 L98 E99 S101 F102 V104 K108 R112 H113 I119 V123 H126 P130 T131 I132 K160 F161 R162 G163 Y320 H323 H324 K331 R335 F346 F347 Y354 A355 I358 K395 Q396 R397 T398 F399 N401 G402 F440 R441 I442 Y444 G447 P448 L449 A450 R451 N453 S454 F456 W458 R461 T466 I467 P469 K504 Y509 T513 N582 R655 M688 H707 K721 Q725 K728 T869 K1048 E1050 V1051 T1053 G1054 G1055 F1056 I1061 P1063 R1073 K1074 K1075 W1077 K1081 Y1082 M1120 H1289 Q1290 Y1296 T1298
BS02	dna	B	D269 Y450 M495 N497 K526 W659 N692 Q695 H698 A728 R778 K902 R925 Q926 K1107 E1108 S1109	D264 Y444 M489 N491 K520 W653 N686 Q689 H692 A714 R754 K878 R901 Q902 K1058 E1059 S1060
BS03	dna	B	K1107 R1114 K1118 V1135 S1136 K1200 S1216 Q1221 A1320 R1333 Q1335 R1337	K1058 R1065 K1069 V1086 S1087 K1151 S1167 Q1172 A1260 R1273 Q1275 R1277

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:5fw1, PDBe:5fw1, PDBj:5fw1
• PDBsum: 5fw1
• PubMed: 26990992
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)