Structure of PDB 5flm Chain B

Receptor sequence
>5flmB (length=1134) Species: 9913 (Bos taurus) [Search protein sequence]
EITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPI
DLQPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVD
ITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNEC
PLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLEN
SSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALG
FVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAK
PGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAAL
GRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGK
DFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFAS
TLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLA
LMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIH
KDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLI
VEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAM
TPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSA
MGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGIN
SIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEE
VFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPTKR
DCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHG
QKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVS
ANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQ
IFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEME
RDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGC
RNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV
3D structure
PDB5flm Structure of Transcribing Mammalian RNA Polymerase II
ChainB
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.48: RNA-directed RNA polymerase.
2.7.7.6: DNA-directed RNA polymerase.
3.1.13.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna B Q468 Q731 R841 H842 R890 K934 K942 H1053 Q441 Q704 R814 H815 R850 K894 K902 H1013
BS02 dna B Q456 K457 D492 L747 R1078 R1085 Q429 K430 D465 L720 R1038 R1045
BS03 ZN B C1119 C1137 C1140 C1079 C1097 C1100
Gene Ontology
Molecular Function
GO:0001055 RNA polymerase II activity
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0003968 RNA-dependent RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0032549 ribonucleoside binding
GO:0046872 metal ion binding
GO:0071667 DNA/RNA hybrid binding
Biological Process
GO:0001172 RNA-templated transcription
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005665 RNA polymerase II, core complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5flm, PDBe:5flm, PDBj:5flm
PDBsum5flm
PubMed26789250
UniProtA5PJW8|RPB2_BOVIN DNA-directed RNA polymerase II subunit RPB2 (Gene Name=POLR2B)

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