Structure of PDB 5f9r Chain B

Receptor sequence
>5f9rB (length=1362) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK
DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLK
RRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMG
RHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVE
NTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSI
DNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK
AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYP
KLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQT
GGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS
LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI
REQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSI
TGLYETRIDLSQ
3D structure
PDB5f9r Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.
ChainB
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna B K33 V34 L35 K44 K45 N46 G56 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 R78 Y81 L101 S104 F105 R115 H116 P117 V126 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 L332 K336 R340 F351 Y359 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 Y515 N588 R661 M694 S730 P731 K735 S777 K918 V922 Q933 T957 V1009 Y1010 G1011 Y1013 M1089 P1090 Q1091 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 Y1131 F1134 M1169 E1225 A1227 Q1272 R1279 H1349 Q1350 S1351 Y1356 T1358 K31 V32 L33 K42 K43 N44 G54 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 R76 Y79 L99 S102 F103 R113 H114 P115 V124 H127 T132 I133 H158 K161 F162 R163 G164 Y323 H326 H327 L330 K334 R338 F349 Y357 I361 Q400 R401 T402 F403 N405 G406 F444 R445 I446 Y448 G451 P452 L453 A454 R455 N457 S458 F460 W462 R465 T470 I471 P473 Y513 N586 R659 M692 S728 P729 K733 S775 K916 V920 Q931 T955 V1007 Y1008 G1009 Y1011 M1087 P1088 Q1089 E1097 V1098 T1100 G1101 G1102 F1103 I1108 P1110 K1111 R1120 K1121 K1122 Y1129 F1132 M1167 E1223 A1225 Q1270 R1277 H1347 Q1348 S1349 Y1354 T1356
BS02 dna B L169 D269 Y450 M495 K526 G582 W659 M694 Q695 H698 R765 E766 Q768 T770 G773 R925 Q926 Y1013 G1030 K1107 E1108 S1109 K1334 L167 D267 Y448 M493 K524 G580 W657 M692 Q693 H696 R763 E764 Q766 T768 G771 R923 Q924 Y1011 G1028 K1105 E1106 S1107 K1332
BS03 dna B G12 T13 N14 E766 G915 F916 Q920 L921 T924 K961 K968 R976 K1107 R1114 K1118 D1135 S1136 P1137 K1200 S1216 Q1221 R1333 R1335 G10 T11 N12 E764 G913 F914 Q918 L919 T922 K959 K966 R974 K1105 R1112 K1116 D1133 S1134 P1135 K1198 S1214 Q1219 R1331 R1333
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:5f9r, PDBe:5f9r, PDBj:5f9r
PDBsum5f9r
PubMed26841432
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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