Structure of PDB 5erg Chain B

Receptor sequence
>5ergB (length=324) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
NCFSGYKDLIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIG
KPYGSQIAIRTFAFVHVLQPTPELWTLSLPHRTQIVYTPDSSYIMQRLNC
SPHSRVIEAGTGSGSFSHAFARSVGHLFSFEFHHIRYEQALEEFKEHGLI
DDNVTITHRDVCQGGFLIKKGDTTSYEFGNNETAASLNANVVFLDLPAPW
DAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQG
RQYESRRQMVRSLNDALERLRDIKRHKIKEGDSNYKWKEVTKMEAEIKSH
TSYLTFAFKVVNRSRDDEKVNEIL
3D structure
PDB5erg Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 from Saccharomyces cerevisiae.
ChainB
Resolution2.202 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.220: tRNA (adenine(58)-N(1))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAM B Q92 V94 G118 G120 S121 S123 F124 E139 F140 R144 R167 D168 V169 D203 L204 P205 Q84 V86 G110 G112 S113 S115 F116 E131 F132 R136 R159 D160 V161 D195 L196 P197 MOAD: Kd=44.33uM
PDBbind-CN: -logKd/Ki=4.35,Kd=44.33uM
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0160107 tRNA (adenine(58)-N1)-methyltransferase activity
Biological Process
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005634 nucleus
GO:0031515 tRNA (m1A) methyltransferase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5erg, PDBe:5erg, PDBj:5erg
PDBsum5erg
PubMed27582183
UniProtP46959|TRM61_YEAST tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 (Gene Name=GCD14)

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