Structure of PDB 5er9 Chain B

Receptor sequence

>5er9B (length=393) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

TGNFDLFVVGSGFFGLTIAERAATQLGKRVLVIERRPHIGGNAYSEPEPE
TGIEVHKYGAHLFHTSNKRVWDYVRQFTDFTGYQHRVFAMHNGQAYQFPM
GLGLVSQFFGRYFSPDEARALIAEQASEIDTKDAKNFEEKAISLVGRPLY
EAFIKHYTAKQWQTDPKDLPASNITRLPVRYTFDNRYFNDTYEGLPVEGY
TKWLENMAADERIEVRLDTDWFDVRDDLRAANPDAPVVYTGPLDRYFDYA
EGRLGWRTLDFELEVLETGDFQGTPVMNYNDLDVPYTRIHEFRHFHPERT
YPTDKTVIMREYSRFADNDDEPYYPINTEADRAVLAAYRARAKAETASAK
VLFGGRLGTYQYLDMHMAIASALSMFDNVLAPHLSEGASLVTE

3D structure

• PDB: 5er9 Structural dynamics of UDP-galactopyranose mutase from Mycobacterium smegmatis
• Chain: B
• Resolution: 1.689 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R189 R193 R270 R301 E324 Y375 D377
• Catalytic site (residue number reindexed from 1): R176 R180 R257 R288 E311 Y362 D364
• Enzyme Commision number: 5.4.99.9: UDP-galactopyranose mutase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UDP	B	F111 I167 Y170 T171 W175 R189 L190 Y200 N291 R301 Y337 Y375	F98 I154 Y157 T158 W162 R176 L177 Y187 N278 R288 Y324 Y362
BS02	FAD	B	V22 G23 G25 F26 F27 I46 E47 R48 R49 G54 N55 A73 H74 L75 D233 W234 F235 T253 G254 P255 R258 G368 R369 D377 M378	V9 G10 G12 F13 F14 I33 E34 R35 R36 G41 N42 A60 H61 L62 D220 W221 F222 T240 G241 P242 R245 G355 R356 D364 M365

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008767 UDP-galactopyranose mutase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5er9, PDBe:5er9, PDBj:5er9
• PDBsum: 5er9
• UniProt: A0R629