Structure of PDB 5emn Chain B

Receptor sequence

>5emnB (length=597) Species: 9606 (Homo sapiens)

SSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEE
YDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGV
KFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF
ITWREQFWLAVCEHFGVEAIRQYELVVHTDIDAAKVYMGEMGRLKSYENQ
KPPFDAKNPFLPAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAV
YPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALT
YYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVE
ARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHIC
AVVVEYETKAGRINKGVATNWLRAKEPLVPMFVRKSQFRLPFKATTPVIM
VGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELA
QFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCG
DARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS

3D structure

• PDB: 5emn Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y459 S460 C632 D677 W679
• Catalytic site (residue number reindexed from 1): Y384 S385 C549 D594 W596
• Enzyme Commision number: 1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	B	S89 Q90 T91 T93 A94 T142 Y143 G144 G177 N178 Y181 F184 N185	S20 Q21 T22 T24 A25 T73 Y74 G75 G108 N109 Y112 F115 N116
BS02	FAD	B	R427 R457 Y458 Y459 S460 C475 A476 Y481 G491 V492 A493 T494 W679	R352 R382 Y383 Y384 S385 C400 A401 Y406 G416 V417 A418 T419 W596
BS03	NAP	B	G537 T538 C569 S599 R600 K605 Y607 Q609 M638	G454 T455 C486 S516 R517 K522 Y524 Q526 M555

Gene Ontology

Molecular Function

• GO:0003958 NADPH-hemoprotein reductase activity
• GO:0005515 protein binding
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

Biological Process

• GO:0006805 xenobiotic metabolic process
• GO:0009725 response to hormone
• GO:0022900 electron transport chain
• GO:0032770 positive regulation of monooxygenase activity
• GO:0090346 cellular organofluorine metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:5emn, PDBe:5emn, PDBj:5emn
• PDBsum: 5emn
• PubMed: 27496950
• UniProt: P16435|NCPR_HUMAN NADPH--cytochrome P450 reductase (Gene Name=POR)