Structure of PDB 5edv Chain B

Receptor sequence

>5edvB (length=333) Species: 9606 (Homo sapiens)

CAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACG
RPDLTDSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCS
FGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDP
EYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYN
AFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMF
NTEPGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINA
HSLTLYEVEELETATERYLHLQKLTEEVPLGQS

3D structure

• PDB: 5edv Structure of a HOIP/E2~ubiquitin complex reveals RBR E3 ligase mechanism and regulation.
• Chain: B
• Resolution: 3.48 Å

Enzymatic activity

• Enzyme Commision number: 2.3.2.31: RBR-type E3 ubiquitin transferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C699 C702 C722 C725	C1 C4 C24 C27
BS02	ZN	B	C719 C744 C747	C21 C46 C49
BS03	ZN	B	C799 C802 C817 C820	C96 C99 C114 C117
BS04	ZN	B	C825 C828 H836 C841	C122 C125 H133 C138
BS05	ZN	B	C871 C874 C890 C893	C168 C171 C187 C190
BS06	ZN	B	C898 C901 H926 C930	C195 C198 H223 C227
BS07	ZN	B	C911 C916 H923 H925	C208 C213 H220 H222
BS08	ZN	B	C969 C986 C998 H1001	C257 C274 C286 H289

Gene Ontology

Molecular Function

• GO:0004842 ubiquitin-protein transferase activity
• GO:0008270 zinc ion binding

Cellular Component

• GO:0071797 LUBAC complex

External links

• PDB: RCSB:5edv, PDBe:5edv, PDBj:5edv
• PDBsum: 5edv
• PubMed: 26789245
• UniProt: Q96EP0|RNF31_HUMAN E3 ubiquitin-protein ligase RNF31 (Gene Name=RNF31)