Structure of PDB 5eaw Chain B

Receptor sequence
>5eawB (length=1046) Species: 10090 (Mus musculus) [Search protein sequence]
MEPLDELDLLLLEAVPRVELLRKKADALFPETVLSRGVDNRYLVLAVETS
QNERGAEEKRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTS
EPWIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRGSDPATRQ
MLIGTILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLSQDE
ILCEVEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGRSSPCNIEVVK
SLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKESNS
IEHRSQVVLYTLLSQERREDPEAGWLLYLKTGQMYPVPANHLDKRELLKL
RNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSR
AVEEDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLESQSKDNRK
THQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPMPA
TNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTV
FRLDREERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFIAYLSS
VLPHDAKDTVANILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTIC
ALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDI
QKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFC
IVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVVNREARALGMSESL
FKRLERNESAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVANAVLA
LPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENG
GVSNVTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLLARSSV
GMVEVNTVDKYQGRDKSLILVSFVRSNEDGTLGELLKDWRRLNVALTRAK
HKLILLGSVSSLKRFPPLGTLFDHLNAEQLILDLPSREHESLSHIL
3D structure
PDB5eaw Dna2 nuclease-helicase structure, mechanism and regulation by Rpa.
ChainB
Resolution3.0 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.1.-.-
3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SF4 B C137 R139 R140 I387 I388 C394 C397 C403 M423 C131 R133 R134 I379 I380 C386 C389 C395 M413
BS02 ADP B L627 Q631 G654 T656 T657 K689 Y828 L617 Q621 G644 T646 T647 K679 Y818
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016887 ATP hydrolysis activity
GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
GO:0017108 5'-flap endonuclease activity
GO:0017116 single-stranded DNA helicase activity
GO:0043139 5'-3' DNA helicase activity
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0000076 DNA replication checkpoint signaling
GO:0000723 telomere maintenance
GO:0000729 DNA double-strand break processing
GO:0006259 DNA metabolic process
GO:0006260 DNA replication
GO:0006264 mitochondrial DNA replication
GO:0006281 DNA repair
GO:0006284 base-excision repair
GO:0032508 DNA duplex unwinding
GO:0033567 DNA replication, Okazaki fragment processing
GO:0043137 DNA replication, removal of RNA primer
GO:0043504 mitochondrial DNA repair
GO:0045740 positive regulation of DNA replication
GO:0090304 nucleic acid metabolic process
GO:1902990 mitotic telomere maintenance via semi-conservative replication
Cellular Component
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005739 mitochondrion
GO:0005760 gamma DNA polymerase complex
GO:0042645 mitochondrial nucleoid

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:5eaw, PDBe:5eaw, PDBj:5eaw
PDBsum5eaw
PubMed26491943
UniProtQ6ZQJ5|DNA2_MOUSE DNA replication ATP-dependent helicase/nuclease DNA2 (Gene Name=Dna2)

[Back to BioLiP]