Structure of PDB 5dqt Chain B

Receptor sequence
>5dqtB (length=279) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
TWLPLNPIPLKDRVSMIFLQGQIDVIDGAFVLIDKTGIRTHIPVGSVACI
MLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALL
AKQYGVTWNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAP
AIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNPGEPDREVRLACR
DIFRSSKTLAKLIPLIEDVLAAGEIQPPA
3D structure
PDB5dqt Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
ChainB
Resolution3.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B D26 V27 D29 S61 D24 V25 D27 S59
BS02 dna B W3 R41 W2 R39
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0005515 protein binding
GO:0008821 crossover junction DNA endonuclease activity
GO:0017108 5'-flap endonuclease activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0006281 DNA repair
GO:0006974 DNA damage response
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus
GO:0099048 CRISPR-cas system
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5dqt, PDBe:5dqt, PDBj:5dqt
PDBsum5dqt
PubMed26478180
UniProtQ46896|CAS1_ECOLI CRISPR-associated endonuclease Cas1 (Gene Name=ygbT)

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